*4.4. P(OEGMA-co-MEO2MA) Thermosensitive Hydrogels for 3D Cell Culture* 4.4.1. Cell Culture

The model cells used in this experiment were human breast cancer cells (MCF-7), which were cultured in a cell culture incubator using DMEM medium. The main components of the culture medium were 90% DMEM high glucose medium, 10% fetal bovine serum, and 100 unit/mL penicillin/streptomycin double antibodies. The culture temperature was 37 ◦C and the carbon dioxide concentration was 5%. *Gels* **2022**, *8*, x FOR PEER REVIEW 11 of 13
