*Article* **Cytochalasans from the Endophytic Fungus** *Phomopsis* **sp. shj2 and Their Antimigratory Activities**

**Bing-Chao Yan 1,2,3 , Wei-Guang Wang 1,2, Ling-Mei Kong 1,2, Jian-Wei Tang 1,2,3, Xue Du 1,2,3, Yan Li 1,2 and Pema-Tenzin Puno 1,2,3,\***


**Abstract:** Cytochalasans from the endophytic fungi featured structure diversity. Our previous study has disclosed that cytochalasans from the endophytic fungus *Phomopsis* sp. shj2 exhibited an antimigratory effect. Further chemical investigation on *Phomopsis* sp. shj2 has led to the discovery of seven new cytochalasans (**1**–**7**), together with four known ones. Their structures were elucidated through extensive spectroscopic data interpretation and single-crystal X-ray diffraction analysis. Compounds **1**–**3** and **8**–**11** exhibited antimigratory effects against MDA-MB-231 in vitro with IC<sup>50</sup> values in the range of 1.01−10.42 µM.

**Keywords:** endophytic fungus; *Phomopsis*; cytochalasan; antimigratory activity
