*Article* **[20(22)***E***]-Lanostane Triterpenes from the Fungus** *Ganoderma australe*

**Lin Zhou 1,†, Li-Li Guo 1,†, Masahiko Isaka <sup>2</sup> , Zheng-Hui Li 1,\* and He-Ping Chen 1,\***


**Abstract:** Twelve new lanostane triterpenoids (**1**–**5**, **7**–**13**) were isolated from the fruiting bodies of the fungus *Ganoderma australe*. The structures of the new compounds were elucidated by extensive 1D and 2D NMR, and HRESIMS spectroscopic analysis. All the triterpenes are featured by 20(22)*E* configurations which are uncommon in the *Ganoderma* triterpene family. The absolute configuration of the C-25 of compounds **1**, **2**, and **6** were determined by the phenylglycine methyl ester (PGME) method. A postulated biosynthetic pathway for compound **1** was discussed. This study opens new insights into the secondary metabolites of the chemically underinvestigated fungus *G. australe*.

**Keywords:** *Ganoderma australe*; triterpene; 20(22)*E* configuration; PGME method
