*4.3. Modification of HSA by Glucose*

HSA glycation was carried out as described previously with minor changes [1,2]. Solutions of HSA (3 mg/mL) with and without glucose were incubated for 10 weeks under identical experimental conditions. To analyze the anti-glycation activity of garlic extract, HSA with glucose was also incubated with varying concentrations of garlic extract (0–100 µg/mL) and incubated under similar conditions. Post-incubation, all samples were dialyzed against PBS. Samples were stored at −20 ◦C. Protein concentrations were determined by NanoDrop™ 2000/2000c spectrophotometer (Thermo Scientific, USA).
