*4.9. Wound Healing Assay*

HUVECs cells were seeded in a 6-well plate and cultured overnight until a confluent monocytic layer was formed and a straight cell scratch was made on the monolayer with the tip of a 200 µL pipette tip. The cells were treated with VEGF or different concentrations of DGS. The scratches were imaged separately using an Olympus IX83 inverted microscope (10×, magnification) (Olympus, Tokyo, Japan). The scratch area was quantified using Image Pro Plus.
