*4.1. Cell and Reagents*

HUVECs were purchased from Jinan Dinguo Changsheng Biotechnology Co., Ltd. (Jinan, China). PTK787 was purchased from the AbMole BioScience (Houston, TX, USA). Pronase E and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (Mannheim, Germany). Coenzyme Q10(CoQ10) was purchased from Yuanye (≥98% HPLC, Shanghai, China). Methyl alcohol and acetonitrile were purchased from TEDIA (Anhui, China). Cell Counting Kit-8 (CCK-8) and bovine serum albumin (BSA) were purchased from GeneView (Houston, TX, USA). Nitric oxide (NO) assay kit was purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). A Human VEGF ELISA kit was purchased from ABclonal (Boston, MA, USA). We used the following primary antibodies: rabbit anti-GAPDH (Cell Signaling; Danvers, MA, USA), rabbit anti-VEGFR2 (Cell Signaling), rabbit anti-Akt (Cell Signaling), rabbit anti-p-Akt (Cell Signaling), rabbit anti-Erk1/2 (Cell Signaling), rabbit anti-p- Erk1/2 (Cell Signaling), rabbit anti-eNOS (Cell Signaling), and rabbit anti-p-eNOS (Cell Signaling). We used the following secondary antibody for the experiment: HRP-conjugated goat anti-rabbit IgG (ABclonal).
