*3.5. Fluorescence Measurements*

The intrinsic fluorescence spectra of the edG were measured in Jasco spectrofluorometer (FP6200) at 25 ± 1 ◦C with a quartz cuvette of 1 cm path. We monitored the changes in the fluorescent emission spectra of the protein in different buffers conditions and in the presence of urea and GdmCl by taking the entrance and exit slits widths at 5 nm and 10 nm, respectively. The protein was excited at 280 nm, and emission spectra were recorded from 300–400 nm in triplicates. The blank values of all the measurements were subtracted from each sample value.
