*4.7. GC-MS Analysis of Plant Extracts*

The ethyl acetate fraction of *A. aspera* aerial part and the *C. gigantea* flower part were analyzed using Shimadzu GCMS-QP2010 Ultra, furnished with a Flame Thermionic Detector (FTD detector), to identify the chemical composition of the fractions. Helium gas was employed as the carrier with a 0.7 mL min−<sup>1</sup> flow rate. The injection temperature was 260 ◦C, and the preliminary column temperature of 100 ◦C was kept for two minutes before ramping to 250 ◦C at a rate of 10 ◦C min−<sup>1</sup> and hold on for 19 min before increasing to 290 ◦C at a rate of 10 ◦C min−<sup>1</sup> . A solvent delay of 3.5 min was used. Mass spectra were documented in the range of 40–650 m/z and compounds were recognized by using NIST11 Library.
