**2. Results and Discussion**

The G protein of RSV plays a vital role in the host–pathogen interaction, which is mediated by pH-dependent or independent pathways [30,32]. Hence, the role of G protein is very crucial for attachment to the host cell receptors. Since all the biological processes are controlled by the pH of the environments, it eventually determines its stability. In this study, we performed the pH-based and chemical-induced denaturation study to examine its effect on the structural and conformational stability of edG in in vitro conditions. Further, we also determined the binding interaction studies of heparan sulfate with edG by fluorescence quenching and in silico approaches.
