4.8.7. Virulent Genes Regulating Network Analysis

A protein-protein interaction network of virulent proteins was established using STRING v11.5 database, accessed on 7 January 2022 (https://string-db.org/) and visualized by using STRING application, available in Cytoscape. A cutoff score of 0.6–0.9 was selected that showed interaction with high confidence. The studied genes were further analyzed by enrichment analysis by setting a significant statistical threshold less than 0.05. The resulting proteins were then classified by four intrinsic factors, such as "betweenness, closeness, radiality and degree" and betweenness, closeness, stress, and degree. The top virulent proteins were ranked using these factors by the CytoHubba application in the CytoScape [61,62].
