*3.4. Absorbance Measurements*

Absorption spectra of edG were measured to detect alteration in the tertiary structure of the protein at a wide range of pH. The spectral measurement was carried out in Jasco UV/visible spectrophotometer (V-660) with a bandwidth of 0.1 and a scan speed of 100 nm/min at 25 ± 1 ◦C, equipped with a water bath for temperature control. The spectra were recorded in the range of 240–340 nm using 0.3–0.6 mg mL−<sup>1</sup> concentration of protein with 1 cm path length cuvettes. All the spectra were recorded in triplicate.
