*2.7. Lipid Peroxidation*

Lipid peroxidation was done by the method of Utley et al. [23]. Briefly, 1 mL of homogenized brain tissue was incubated in a metabolic shaker at 37 ◦C for one hour, 1.5 mL of 20% TCA was added, which was then centrifuged at 600 g for 10 min. Next, 1 mL of supernatant was added to 1 mL of freshly prepared Thiobarbituric acid (0.67%). The reaction was kept in the water bath for 10 min. Upon cooling, absorbance was read at 535 using a reagent blank. Values were expressed as nanomoles of malondialdehyde formed hour−<sup>1</sup> mg protein−<sup>1</sup> .
