*4.4. Total Flavonoid Content (TFC)*

The TFC of the medicinal plant extracts was determined by the aluminum chloride calorimetric method [39]. A 100 µL solution of 2% aluminum chloride in methanol was added to 100 µL of extract samples. The solution was incubated for 30 min at room temperature (RT) and the optical density was measured at 415 nm. Before adding the aluminum chloride solution, a pre-plate reading was taken. The standard curve was built using five different Rutin concentrations. Extract TFC was measured in mg Rutin equivalents per gram of extract [40].
