*2.5. Detection of Total Polyphenolic Content (TPC) of Plants*

Polyphenolic compounds are key constituents, known to protect plants from reactive oxygen species. This feature enables the extracts to act as reducing agents and as free radical scavengers [39,40]. The total phenolic content (TPC) of *A. aspera* (aerial and root), *C. procera*, *C. gigantea* and *C. gigantea* ash parts were analyzed in water, methanol, ethyl acetate, ethanol, and hexane fractions, shown in Figure 2. The highest TPC was found in the ethyl acetate fraction of *A. aspera* i.e., 15.1 <sup>±</sup> 0.36 mg GAE g−<sup>1</sup> and 18.27 <sup>±</sup> 0.56 mg GAE g−<sup>1</sup> in the aerial and root parts, respectively. *C. gigantea*, *C. gigantea* ash and *C. procera* showed the highest TPC measures (in mg GAE g−<sup>1</sup> ) in the ethanol fraction (36.47 ± 0.93), the ethyl acetate fraction (37.45 ± 0.94), and the ethyl acetate fraction (25.85 ± 0.22), respectively.
