**5. Conclusions**

Previous researchers have demonstrated that there is a complex network of interdependencies and correlations among qualities of various natural products, which may work together to provide the overall therapeutic capabilities of different medicinal plants. The results show that the inhibition of heat-induced denaturation of protein and prevention of glycation and AGEs formation increased with an increase in garlic extract concentration. Furthermore, garlic extract exhibited significant levels of protection in protein structural stabilization against glycation. Our research significantly supports the numerous therapeutic properties of garlic and addresses the question of the interdependence of various biological activities and their antioxidant capacity. Thus, it is suggested to include garlic into any existing preventative and treatment approach for glycation-induced health complications in diabetic patients. For this purpose, a well-designed research strategy involving animal

and clinical study is highly recommended for the verification of the therapeutic advantages of garlic extract.

**Author Contributions:** Conceptualization, M.W.A.K., A.A.O. and S.S.; methodology, M.W.A.K., A.A.O., S.S., W.A.K., R.S. and S.R.V.; validation, N.A., A.K.D.A. and W.A.K.; formal analysis, M.W.A.K., A.A.O., S.S., R.S. and S.R.V.; investigation, M.W.A.K., A.A.O., S.S. and W.A.K.; resources, M.W.A.K., A.A.O., S.S., S.A.A.-Z., E.M.A. and W.A.K.; data curation, M.W.A.K. and A.A.O.; writing original draft preparation, M.W.A.K. and S.S.; writing—review and editing, N.A., E.M.A., S.A.A.-Z. and A.K.D.A.; supervision, M.W.A.K.; funding acquisition, M.W.A.K. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research has been funded by the Scientific Research Deanship at the University of Ha'il–Saudi Arabia through project number RG-20 120.

**Institutional Review Board Statement:** This study was reviewed and approved by the Research Ethics Committee (REC) at the University of Hail dated 27/11/2020 and approved by university president letter number Nr. 20455/5/42 dated 16/04/1442 H.

**Informed Consent Statement:** Not applicable.

**Data Availability Statement:** The data presented in this study are available on request from the corresponding author.

**Acknowledgments:** Authors are highly thankful to the Molecular Diagnostic and Personalised Therapeutics Unit, University of Ha'il, Ha'il 2440, Saudi Arabia, for providing resources and support for the completion of this research.

**Conflicts of Interest:** The authors declare no conflict of interest.

**Sample Availability:** Samples of the compounds are not available from the authors.
