*4.8. Determination of the Target Proteins: Using In Silico Approaches*

To study the mycobacterial target, the proteins of virulence, detoxification, adaptation (VDA) functional category and proteins having PDB structures from the mycobacterial database were selected and an array of in silico analysis was performed. The mycobacterial genome database Mycobrowser was analyzed for the availability of genes responsible for mycobacterial virulence and categorized into virulence, detoxification, adaptation category [43]. *M. tuberculosis* H37Rv genome had a total 4173 proteins, out of which 238 proteins belonged to virulence, detoxification, adaptation category. On the other hand, the RSCB-PDB databank contained PDB structures of 135 *M. tuberculosis* H37Rv proteins excluding repeated or mutated ones, which were categorized in the mentioned categories (Figure S1). The co-integrative analysis of both these categories showed that there were 10 VDA functional category proteins, having PDB structures that were also present, and these 10 proteins were, therefore, present in both categories. We employed various bioinformatics tools which might empower experimental work to identify the prospective targets of this bacterium and illuminated the efficacy of significant regulators of mycobacterial pathways (Table S2) [44].
