*2.8. Assessment of Multitarget Signature Mtb Proteins: An In-Silico Approach*

Mycobrowser was analyzed for the availability of genes or proteins responsible for mycobacterial virulence and these were categorized into virulence, detoxification, and adaptation categories [43]. The Venn-diagram used to categorize V.D.A category and known *Mtb* PDB structure proteins is provided in the supporting material (Figure S1). The PDB structures were assessed by employing different in silico methods (Table 3). The crystal structures of the selected *M. tuberculosis* H37Rv proteins (PDB: 7LD8, 4Q4N, 5XE2, 4LJ1, 1TQ8, 4CHG, 5WZ4, 2JAX, 5SV2, 6L2A) [44] are provided in Figure S2.

**Table 3.** Assessment of multitarget signature *Mtb* proteins based on virulence, detoxification, and adaptation category.



**Table 3.** *Cont.*

**Figure 3.** GC-MS chromatogram of ethyl acetate fractions of (**a**) *A. aspera* (aerial) and (**b**) *C. gigantea* (flower ash). TIC\* indicates Total Ion Chromatogram.
