3.4.2. Steady-State Fluorescence Measurements

The intrinsic fluorescence spectra of HSA were recorded at an emission wavelength (300–420 nm) upon excitement at 295 nm. The HSA (5 µM) samples were titrated with QTP (0–35 µM) at three different temperatures (295, 300, 305 K) to estimate thermodynamic parameters. The obtained fluorescence data were corrected for inner filter effects.
