3.4.4. Competitive Site Probe Displacement (CSPD) Experiments

Briefly, in these experiments, CSPD experiments were carried out to locate the binding site of QTP on the HSA. Warfarin (WAR) (Sudlow's site I), ibuprofen (IBU) (Sudlow's site II), and hemin (HEM) (site III) site markers were used to locate the binding region of QTP in HSA. Initially, fluorescence spectra were performed by titrating a solution of 5 µM HSA and QTP 10 µM with increasing site marker (0–30 µM) concentrations in separate experiments. All other parameters (excitation and emission wavelength) were uniform for the fluorescence measurements.
