*4.5. Total Polyphenolic Content (TPC)*

The TPC of the medicinal plant extracts was estimated by slightly changing the Folin-Ciocalteu method used by Siddhuraju et al. [39]. A pre-plate reading was taken and after that 20 µL of each plant extracts were added to 110 µL of ten times diluted newly made Folin-Ciocalteu reagent. After that, 70 µL of sodium carbonate solution was added and incubated for 30 min at RT and the optical density (absorbance) was determined at 765 nm. Gallic acid (GA) was used as a standard to plot standard curves with five different concentrations. The medicinal plant extract TPC was quantified in milligram (mg) of GA equivalents per gram (g) of extract [40].
