*3.6. Carbonyl Content*

Glycation is a non-enzymatic reaction involving protein and sugar. It results in the formation of an unstable Schiff base, further leading to ketoamine production [33]. HSA was incubated with MG and was studied for its carbonyl content. Native HSA and MGincubated HSA showed differences in their concentration by more than double. Native HSA was estimated 1.12 nmol/mg, while glycated HSA had 2.17 nmol/mg carbonyl content. With successive addition of caffeic acid and coumaric acid, carbonyl content decreased slightly (Figure 5C, Tables 3 and 4).
