*2.4. Synchronous Fluorescence Spectroscopic (SFS) Studies*

The microenvironmental changes in the fluorophore residues were identified with the SFS technique. The changes in the amino acid residues Tyr (tyrosine) and Trp (tryptophan) can be observed at ∆λ = 15 nm and ∆λ = 60 nm. A shift in the emission spectra indicates changes in the polarity of amino acid residues around the fluorophore molecules.
