*4.12. VEGF and NO Level Detection*

Cultures of drug-treated HUVECs cells were collected separately, and the supernatant was removed through centrifugation at 10,000 rpm and then stored at −20 ◦C. The VEGFA expression was assessed using an ELISA kit (R&D), as per the manufacturer's institutions. The amount of NO secreted was enumerated according to the manufacturer's instructions.
