*2.5. Three-Dimensional (3D) Fluorescence Spectroscopy*

Structural changes and conformational changes in the protein are identified with 3D fluorescence spectra. In addition, microenvironmental fluctuations in the vicinity of fluorophore residues lead to conformational or structural changes in the protein molecules. The BSA-ERL 3D spectra were matched to the 3D spectra of BSA to identify any such variations in the fluorophore microenvironment.
