*2.6. Minimum Inhibitory Concentrations (MICs)*

Extract codes 3 and 23 of the ethyl acetate fractions of *A. aspera* aerial and *C. gigantea* flower ash were found to be active against the *M. tuberculosis* H37Rv ATCC 27294 strains, respectively, having an MIC value of 64 mg/L. The rest of the extracts were inactive against tuberculosis and non-tuberculosis strains and showed the MIC value > 64 mg/L. None of the compounds screened were active against non-tuberculous *Mycobacterium*. The known anti-tuberculosis drugs for virulent strains, such as isoniazid, rifampicin, streptomycin, ethambutol showed significant MIC against *M. tuberculosis* H37Rv, and levofloxacin (drug used for avirulent strains) showed significant MIC values against all the tested avirulent strains [41,42] (Table S1).

**Figure 2.** Total Flavonoid content determination (**a**) *A. aspera* (**b**) *C. gigantea* and *C. procera*.
