**2. Materials and Methods**

Experiments were performed on Sprague Dawley rats from the colony bred at the Animal House of Universidad Autónoma de Madrid (ES-28079-0000097). This study was performed in accordance with European Union Guidelines for the Care and Use of Laboratory Animals (Directive 2010/63/EU and Spanish RD 1201/2005) and was approved by the Ethics Review Board of Universidad Autónoma de Madrid and by the local government (Comunidad Autónoma de Madrid, Spain; PROEX 004-19, approved on 7 May 2019).

The rats were housed in buckets 36.5 × I21.5 × 18.5 cm (length×width×height) on aspen wood bedding, maintained under controlled conditions of temperature and 12/12 light/dark photoperiod, and the welfare of the animals was monitored daily by staff. The animals were fed with a EuroRodent breeding diet (5LF5; Labdiet, Madrid, Spain) containing 22.0% protein, 4.4% fat, 55.0% carbohydrates, 4.1% fiber, and 5.4% mineral (sodium content 0.26%) and drinking water were provided *ad libitum*.
