*2.1. Tissue Collection*

DNA barcode sequences published as part of this data release comprise collections conducted from two GGI-Gardens partners—the Botanical Research Institute of Texas (BRIT) and the Missouri Botanical Garden (MOBOT) between 2017 and 2020. A total of 817 collections (from 788 species) are represented in this data release, and these include 174 families and 702 genera (Supplementary Table S1). All collections were conducted following published best practices [2] and include herbarium vouchers, as well as genomic vouchers that include tissue preserved in silica gel and flash frozen in liquid nitrogen. Collections were prioritized using the GGI Gap Analysis Tool (https://globalgeno.me, accessed on 30 January 2022) following the scheme proposed in Linsky and Gostel [19] and whether DNA barcode quality sequences were available in GenBank. Silica-dried leaf tissues were sampled to generate DNA barcode sequences published in this study.
