*2.5. Local Barcode Reference Database Construction*

A local reference database was created using ITS1, ITS2, *trnL-F*, and 5--ETS sequences of the reference Poaceae species from herbarium and field samples Sanger sequenced at the Evrogen company (Moscow, Russia). Sanger-sequenced barcode sequences were trimmed from both ends by the quality and aligned using MAFFT v7.490 (FFT-NS-I algorithm). In addition, sequences of the studied DNA barcodes of the reference species have been retrieved from the GenBank database (if available) and added to the alignment if sequences overlapped and showed similarity by more than 90% with our sequences. Detailed information on the corresponding MSU Herbarium voucher numbers, field samples, and the GenBank accessions can be found in Supplementary Table S1. All barcode sequences were used to construct a local reference BLAST database as described elsewhere [37].
