*2.1. Newly Generated Sequence Data from Fresh and Historical Collections* 2.1.1.Sampling

Our entire dataset included 1130 unique samples (Table S1), 62 were new for this study (or "fresh specimens" from 2016) and 274 represented "historical specimens" (collected between 1888 and 1998, i.e., at least more than two decades ago). The majority of the contemporary samples (remaining 794 samples in the dataset) were collected during field trips between 2007 and 2018 throughout Mexico, Central and South America, and the Caribbean. The historical samples analyzed here are all deposited at the US Herbarium of the Smithsonian National Museum of Natural History. Contemporary samples are deposited at multiple herbaria (mainly B, CDS, CR, F, GMUF, UDBC, US; but also at CUVC, EB-BUAP, FAUC, KRAM, LPB, QCNE, UPR).
