*Article* **Graded Moisture Deficit Effect on Secondary Metabolites, Antioxidant, and Inhibitory Enzyme Activities in Leaf Extracts of** *Rosa damascena* **Mill. var.** *trigentipetala*

**Kamel Hessini 1,\*, Hanen Wasli <sup>2</sup> , Hatim M. Al-Yasi <sup>1</sup> , Esmat F. Ali <sup>1</sup> , Ahmed A. Issa <sup>1</sup> , Fahmy A. S. Hassan <sup>3</sup> and Kadambot H. M. Siddique <sup>4</sup>**


**Abstract:** Drought affects plant growth and yield in many agricultural areas worldwide by producing negative water potentials in the root zone that reduce water availability, affecting plant development and metabolism. This study investigated the effect of varying moisture regimes (100% field capacity (FC), well-watered plants, 50% FC (moderate water stress), and 25% FC (severe water stress)) on growth parameters, chlorophyll content, and bioactive molecule patterns, and the impact on antioxidant, lipoxygenase (LOX), and acetylcholinesterase (AChE) activities in *Rosa damascena*. The water deficit treatments reduced biomass production for both treatments (−29 and −33%, respectively, for MWS and SWS) and total chlorophyll (−18 and −38% respectively for MWS and SWS), relative to the control. The 50% FC treatment had the greatest effect on the phenolic profiles and their respective functionalities, with significant increases in the levels of total phenolic, benzoic (gallic, *p*-coumaric, and syringic acids) (+32%), and cinnamic (caffeic and *trans*-cinnamic acid) acids (+19%) and flavonoids (epicatechin-3-*O*-gallate) (+15%) compared to well-watered leaves (control leaves). The 50% FC treatment also exhibited the highest potential antioxidant activities (apart from NO-quenching activity), evidenced by the lowest IC<sup>50</sup> and EC<sup>50</sup> values. The inhibitory LOX and AChE capacities varied depending on the severity of stress, with superior activity in the 50% FC treatment. Overall, the drought tolerance in rose was associated mainly with its suitable manipulation of antioxidant production and orderly regulation of LOX and AChE activities.

**Keywords:** AChE activity; antioxidant; drought stress; LOX activity; phenolics; damask rose
