• In vivo kinetics of recombinant viruses

Four chickens per group were euthanized at 7, 14, 28, and 35 dpi, and the blood, spleens, and feather tips were collected. Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation using Percoll solution (GE Healthcare, Chicago, IL, USA). DNA was extracted from the PBMCs, spleens, and feather tips, and the viral loads were analyzed for each sample.
