*4.8. Statistical Analysis*

Data are expressed as mean values ± SEM, indicating *n* as the number of single measurements, and N is the number of independent experiments, which means independent seeding of cells. Statistical analysis was performed using Student's *t*-test between the tricellulin KD clones and their corresponding control (KD 11 and KD 17 versus control 12). In case of angulin-1 KO clones, statistical significance of the difference between means was evaluated using one-way ANOVA and the Tukey and Dunnett adjustment for multiple testing.

MDCK C7 cells: \* *p* ≤ 0.05, \*\* *p* ≤ 0.01, \*\*\* *p* ≤ 0.001 with regard to control 14 and # *p* ≤ 0.05, ## *p* ≤ 0.01, ### *p* ≤ 0.001 with regard to control 18. HT-29/B6 cells: \* *p* ≤ 0.05, \*\* *p* ≤ 0.01, \*\*\* *p* ≤ 0.001 with regard to control 15 and # *p* ≤ 0.05, ## *p* ≤ 0.01, ### *p* ≤ 0.001 with regard to control 29. Statistical analysis was performed using GraphPad Prism 8 software (GraphPad; San Diego, CA, USA). A probability value of *p* ≤ 0.05 was considered statistically significant.
