**Appendix A**

**Figure A1.** The survival rates after the grouping. There is no significant difference in the survival rate after the grouping among the three groups (control group, 80%; aspirin group, 100%; cilostazol group, 69% (*p* = 0.109, log-rank test: 14 mice evaluated for the first specimen are excluded from the analyses). Even in the two group comparisons, no differences are seen in the survival rate between the control group and the aspirin or cilostazol group (*p* = 0.098 and *p* = 0.550, log-rank test).

**Figure A2.** A representative image of accumulation of erythrocytes or hemosiderin. Accumulation of erythrocytes or hemosiderin shown in a Tg2576 mouse (aged 21 months old) fed with aspirin (i.e., aspirin group) (H&E stain). (**a**) Small accumulations of erythrocytes (arrow heads) are found around a leptomeningeal artery with lymphocytic infiltration (arrow). (**b**) Hemosiderin depositions (arrow heads) are seen around a leptomeningeal artery with vasculitis (arrow). Scale bars indicate 50 μm.

**Scheme A1.** Semi-automatic measurement of senile plaques in the regions of interest (cortex and the hippocampus). Upper line: Measurement of the area of the section: (1) Filling of the section (**a**) with red color (**b**); (2) dichotomization of color into black and white using a semiautomatic method with an appropriate color threshold (**c**). Lower line: Measurement of the total area of the senile plaque: (1) digital stripping of Aβ-stained lesions located out of the regions of interest (**d**); (2) change of color of the remaining Aβ-stained area to red using manual calibration with an appropriate color threshold (**e**); (3) dichotomization of color to black (Aβ-stained lesions in regions of interest) and white (other) using a semiautomatic method with an appropriate color threshold (**f**).

#### **References**


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