*2.3. Structural Characterization of Alg*−*Chi Nanoparticles*

Scanning electron microscopy (SEM) was used to explore the morphology of the Alg−Chi NPs with a Quanta field emission gun (Model 650) operated at 20.0 kV. Images were obtained in secondary electron mode.

To determine the size of the Alg−Chi obtained NPs, dynamic light scattering (DLS) measurements were used with a Zetasizer ZS90 (Malvern) equipped with a helium-neon gas laser with a wavelength of 632 nm.

Fourier transforms infrared (FTIR) measurements were used to verify the presence of the polymers in the synthesized Alg−Chi NPs, which identified the presence of the functional groups of each compound. A Bruker Tensor II spectrometer equipped with a platinum ATR cell and a cooled deuterated triglycine sulfate (DTGS) detector were used.
