*2.7. Data Analysis*

#### 2.7.1. Sample Size and Power

A sample size calculation for generalized linear mixed effects models (GLMMs) through simulation [42] in R statistical software 3.02 https://www.r-project.org/ (accessed on 23 April 2022) was performed to detect a 10% effect difference between the nets, simulations were performed using an estimated mosquito mortality of 80% for unwashed Interceptor® G2 and 70% for unwashed Interceptor®, and 10% for SafiNet® (deliberately holed). The power estimated was more than 90% based on estimates from previous studies conducted in the same setting: mean mortality of 81.5% for the WHO tunnel test with an assumed daily variation of 0.5 and 15 replicates per arm [23].
