4.8.2. Total Protein Quantification

The remaining tissue homogenate was centrifuged for 20 min at 12,000 rpm (4 ◦C) to obtain the post-mitochondrial supernatant fraction (PMS). Total proteins in homogenates were measured by using Pierce™ BCA Protein Assay Kit, as described by the manufacturer (ThermoFisher Scientific, Waltham, MA, USA).
