*4.4. Fish Rearing Conditions and Feeding Scheme*

The experiment was carried out in compliance with the Guidelines of the European Union Council (Directive 2010/63/EU) and Portuguese legislation for the use of laboratory animals at CIIMAR aquaculture and animal experimentation facilities in Matosinhos, Portugal. The protocol was approved by the CIIMAR Animal Welfare Committee in 29/04/2020 with the reference 0421/000/000/2020 from Direção Geral de Alimentação e Veterinária (DGAV). Seawater flow was kept at 4 L min−<sup>1</sup> (mean temperature 22.4 ± <sup>1</sup> ◦C; mean salinity 35.2 ± 0.7 ‰) in a recirculation system with aeration (mean dissolved oxygen above 6 mg L−1). Water quality parameters were monitored daily and adjusted when necessary. Mortality was monitored daily. Diets were randomly assigned to triplicate groups of 97 fish/tank (IBW: 33.4 ± 4.1 g) that were fed to satiation three times a day for 2 weeks starting at a 1.5% biomass.
