Blood Analysis Study 1

Blood samples were collected after an overnight fast into EDTA tubes by qualified phlebotomists from a professional pathology service supplier (Southern Pathology IML). Fasted blood samples were analyzed for total cholesterol, triglycerides, non-HDL cholesterol, LDL-cholesterol, CRP, glucose, C-peptide, and insulin. Samples were analyzed using a Roche Cobas 8000 or Roche Cobas Pro by Southern Pathology IML. Total cholesterol was measured using the cholesterol oxidase/peroxidase method, triglycerides were measured using the lipase/glycerol kinase method, non-HDL and LDL cholesterol were measured using the dextran sulfate/polyethylene glycol modified enzymes method, CRP was measured using a turbidimetric assay, glucose was measured using the hexokinase method, and C-peptide and insulin were measured using an electrochemiluminescence assay.

Further blood-count variables were measured for safety context and are provided in the Supplementary Material (Table S4).
