4.7.3. Nitric Oxide (NO) Production

NO production was measured in plasma (1:10 sample dilution) and intestine (1:5 sample dilution) samples. Total nitrite and nitrate concentrations in the sample were assessed using the Nitrite/Nitrate colorimetric method kit (Roche, Basel, Switzerland) adapted to microplates. Nitrite concentration was calculated by comparison with a sodium nitrite standard curve. Since nitrite and nitrate are endogenously produced as oxidative metabolites of the NO molecule, these compounds are considered as indicative of NO production.
