*4.8. Analysis of Liver Function*

The blood samples were collected as previously described [94,95]. Blood samples were gained by removing the eyeballs of mice. Blood was then centrifuged at 4000 rpm for 30 min at 4 ◦C. The samples were incubated in an electro-thermostatic water bath at 37 ◦C for 30 min. The serum was collected and subjected to the examination of the activities of alanine transaminase (ALT), aspartate aminotransferase (AST) activities, the activities of lactate dehydrogenase (LDH), and alkaline phosphatase (ALP) with respective commercial kits. The determination of AST, ALT, LDH, and ALP was performed by the instruction of the kits (Nanjing Jianchen Bioengineering Institute, Nanjing, China).
