*4.6. FJB Staining*

FJB stains all degenerating neurons regardless of specific insult or mechanism of cell death. FJB staining was performed, as described previously [37]. In short, the sections were soaked in 0.06% potassium permanganate and incubated in 0.0004% FJB (Cell Signaling Technology, Beverly, MA, USA). Thereafter, these sections were washed and put on a hot plate (about 50 ◦C) for the reaction of FJB. Finally, the sections were cleared in xylene and coverslipped with DPX (Thermo Fisher Scientific, Waltham, MA, USA).
