*4.13. Statistical Analysis*

The experiments were performed on a minimum of three independent determinations, carried out in triplicate, and the results are reported as the means ± SD.

For comparison between the two groups, a *t*-test was used, and differences were considered to be significantly different if *p* < 0.05 (GraphPad PRISM 8.2). One-way analysis of variance (ANOVA) was carried out in GraphPad PRISM 8.2 software to evaluate any statistical difference among more than two different samples. Differences were considered to be significantly different if *p* < 0.05 (Tukey's post-hoc multiple-comparison test).
