**4. Materials and Methods**

#### *4.1. Reagents*

Reagents for cell culture were obtained by Euroclone (Euroclone, Italy). All chemical reagents were purchased by Sigma Aldrich (Sigma, St. Louis, MO, USA). 2- ,7- - Dichlorodihydrofluorescein diacetate (H2DCFDA) was supplied by Invitrogen (Invitrogen, Carlsbad, CA, USA).
