*4.12. Measurement of Bax, Caspase-3 and Bcl-2 in Hepatic Tissue*

The levels of pro-apoptotic-related protein Bax (MM-1143H2) and anti-apoptoticrelated protein Bcl-2 (MM-0306M2) were performed using an ELISA kit (MeiMian, Yancheng, Jiangsu, China) according to the manufacturer's instructions. Caspase-3 activity was measured using a kit (C1115) purchased from Beyotime Biotechnology (Shanghai, China).
