*4.9. Measurement of MDA, SOD, CAT and GSH-Px Activities*

The changes in hepatic oxidative stress were monitored as previously described [65]. The liver homogenate was centrifuged to obtain the supernatant at 3500 rpm for 10 min at 4 ◦C, and the superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) activity, and malondialdehyde (MDA) levels were measured according to the manufacturer's instructions.
