4.5.1. Health Status (Experiment One)

After 1 and 2 weeks, 12 fish/treatment were weighed and sampled for tissues (blood, head-kidney, liver and gut), after being sacrificed with a 2-phenoxyethanol lethal dose (0.5 mL L<sup>−</sup>1) [55]. Blood was collected from the caudal vein using heparinised syringes and centrifuged at 10,000× *g* for 10 min at 4 ◦C to obtain plasma samples. Plasma and tissue samples were immediately frozen in liquid nitrogen and stored at −80 ◦C until further analysis.
