*4.5. Nissl Staining*

Nissl staining is a widely used method to study the morphology and pathology of neural tissue. To examine IR injury in the gerbil hippocampus, Nissl staining was performed in the usual way. Briefly, the sections were submerged in 0.1% CV acetate solution for 20 min at room temperature. These sections were briefly washed in distilled water, dehydrated in graded ethanol and then cleared in xylene. Lastly, they were mounted with Canada balsam (Sigma-Aldrich).
