*4.12. [111. In]In-IP-001 Cellular Uptake*

A549 epithelial lung cancer cells were seeded in 6-well plates (20,000/cm<sup>2</sup> ) and allowed to adhere overnight. The following day, the medium was removed and radiotracer

uptake was studied by incubating 2 <sup>×</sup> <sup>10</sup><sup>5</sup> cells at 37 ◦C in 2 mL of culture medium added to 30 µL (ca. 2 MBq, 1.35 µg, 1 nmol) of [111In]In-IP-001. Uptake was monitored at 2, 4, 8, and 24 h. At these time points, the medium was removed and cells were washed twice with 2 mL of ice-cold PBS. Finally, cells were detached with 2 mL of a 0.25% trypsin/EDTA solution at 37 ◦C and centrifuged to separate the supernatant from the cells pellet. The radioactivity associated with the pellets was measured in a γ-spectrometer and corrected for decay. All experiments were performed in triplicate.
