*2.1. General*

A commercially available sterile, single-use cassettes (produced according to GMP) used for <sup>68</sup>Ga-cationic prepurification (catalog number 10886) were purchased from TRASIS and adapted for the <sup>89</sup>Zr purification. PEEK tubing and PEEK connectors were purchased separately from IDEX Health & Science, LLC (Middleboro, MA, USA) and sanitized with hydrogen peroxide solution and dried using nitrogen gas. A customized glass target dissolution vial with a vented glass cover that also allowed the insertion of PEEK tubing for the addition and removal of solutions into the vial and a aluminium heating block for the vial were manufactured at the University of Saskatchewan (Saskatoon, SK, Canada). All commercially obtained chemicals were of the highest available purity grade and were used without further purification. Hydrochloric acid (99.999% trace metal basis), water (Omni *Trace* Ultra™), oxalic acid (99.999% trace metals basis), acetonitrile (99.999% trace metal basis), *N*-(3-dimethylaminopropyl)-*N*¢-ethyl carbodiimide hydrochloride (99.9%), hydroxylamine hydrochloride (99.999% trace metal basis), 2,3,5,6 tetrafluorophenol (97%), diethylenetriaminepentaacetic acid (99%), 1.5 mL Eppendorf® Safe-Lock microcentrifuge tubes, and empty reversible SPE tubes (0.5 mL) were purchased from Sigma Aldrich (Oakville, ON, Canada). Methanol (semiconductor grade) was purchased from Fisher Scientific (Ottawa, ON, Canada). *p-SCN-Bz*-DFO was purchased from Macrocyclics, Inc. (Dallas, TX, USA). PEEK tubing 1/16" OD × .040" ID, flangeless nut PEEK, Short, 1/4-28 flat-bottom, for 1/16" OD, flangeless ferrule tefzel, (ETFE), 1/4-28 flat-bottom, Luer-adapter assembly 1/4-28 female–male PEEK, and Luer-adapter 1/4-28 female to female Luer PEEK, were obtained from IDEX Health & Science, LLC (Middleboro, MA, USA). Sep-Pak Accell Plus CM light cartridges containing weak cation-resin were purchased from Waters (Milford, MA, USA). A tube rotator was purchased from VWR International (Mississauga, ON, Canada). Instant thin-layer chromatography paper (iTLC) was purchased from Agilent Technology (SantaClara, CA, USA). The radio-TLC plates were scanned using a Bioscan AR-2000 radio-TLC scanner (Washington, DC, USA). Radioactivity was measured using a Capintec CRC-55tr dose calibrator (Florham Park, NJ, USA) or a 1470 Wallac Wizard automated gamma counter (Perkin Elmer, Ramsey MN, USA).
