3.3.4. Polymer Precipitation

Another technique for EV isolation is a method based on polymer precipitation, commonly used to precipitate other molecules such as viruses. In this method, the sample containing the EVs is precipitated with a solution of polyethylene glycol (PEG). Then, with a centrifugation, it is possible to obtain a pellet containing EVs [1]. This approach for EVs isolation is easy, scalable and does not require long runs or specific equipment. Actually, different commercial EV isolation kits, such as Exosome Isolation (Thermo Fisher Scientific) or ExoQuick (System Biosciences), are used to simplify the EVs isolation process [40]. However, the purity of the sample obtained this way is not currently suitable for clinical application [40,77].
