3.3.5. Microfluidic Separation

Finally, microfluidics is a separation method widely used for other nanoparticles. This method is fast and presents high sensitivity as it can be combined with other techniques such as immunoaffinity methodologies, using surface protein markers to enrich the sample of exosomes without contamination [1,41,72]. This technique allows one to obtain relatively pure samples of EVs. For instance, CD41-positive platelet-derived EVs were isolated from plasma through a quick process using an anti-CD41 antibody-coated surface [33]. However, a strong limitation of this method is the low sample volume restriction [1,72].
