*Article* **The Infectious Bronchitis Coronavirus Pneumonia Model Presenting a Novel Insight for the SARS-CoV-2 Dissemination Route**

**Ekaterina Nefedova <sup>1</sup> , Vyacheslav Koptev <sup>1</sup> , Anna S. Bobikova 1,2, Viktoria Cherepushkina <sup>1</sup> , Tatyana Mironova 1,2, Vasily Afonyushkin 1,3, Nikolai Shkil <sup>1</sup> , Nikolai Donchenko <sup>1</sup> , Yulia Kozlova <sup>3</sup> , Natalia Sigareva <sup>2</sup> , Natalia Davidova <sup>1</sup> , Nina Bogdanchikova 4,\* , Alexey Pestryakov <sup>5</sup> and Yanis Toledano-Magaña 6,\***


**Abstract:** Infectious bronchitis (IB) of chickens is a highly contagious disease characterized by damage of the respiratory system and reproductive organs in young animals caused by a virus of the genus Gamma coronavirus. The condition of the respiratory system caused by the IB virus in chickens has many similarities with the pathology of the respiratory system caused by SARS-CoV-2 in humans. The effectiveness of virucidal drugs (Argovit, Triviron, Ecocid, and lauric acid monoglyceride) was tested on chickens inoculated with a tenfold dose of a vaccine strain based on the attenuated virus H120 against IB of chickens. On the 6th day after inoculation, inflammatory changes in the intestines, lungs, and thymus were observed in the control group. The experimental groups were characterized by less pronounced inflammatory reactions and a lower proportion of thymus and lung probes containing genomic IB virus RNA. Since the virucidal activity of four orally administrated formulations was possible only in the intestine, the experimental data indirectly confirmed the hypothesis of the possibility of the predominant accumulation of coronaviruses in the intestine and subsequent lung damage due to the hematogenous redistribution of viral particles and IBV antigens. It was suggested that other coronaviruses including SARS-CoV-2 can implement a similar mechanism.

**Keywords:** virucidal drugs; IBV; chickens; Coronaviridae; silver nanoparticles; artificial ribonuclease; monoglyceride lauryl acid
