3.3.4. VAS Analysis

Tables 6 and 7 show the VAS results. The amount of change in tension from before Uchida–Kraepelin stress loading to 60 min after Uchida–Kraepelin stress loading in week 8 was 0.86 ± 1.67 in the placebo intake group, −0.41 ± 1.61 in the 500 mg *Euglena* intake group, and −0.29 ± 1.43 in the 3000 mg *Euglena* intake group, demonstrating a reduction in tension (*p* = 0.097, *p* = 0.099, Steel's test). The degree of change in tension from before Uchida–Kraepelin stress loading to 60 min after Uchida–Kraepelin stress loading in week 12 was 0.91 ± 1.77 in the placebo intake group and −0.74 ± 1.51 in the 1000 mg *Euglena* intake group, indicating a significant easing of tension (*p* = 0.023, Steel's test).



\*\*\* *p* < 0.001, \*\* *p* < 0.01, \* *p* < 0.05, † *p* < 0.1 vs. the placebo group. POMS2: Profile of Mood States 2nd Edition.







\*\*\* *p* < 0.001, \*\* *p* < 0.01, \* *p* < 0.05, † *p* < 0.1 vs. the placebo intake group. VAS: visual Analog Scale.

The amount of change in irritability from before Uchida–Kraepelin stress loading to immediately after Uchida–Kraepelin stress loading in week 12 was 1.79 ± 2.00 in the placebo intake group and 0.27 ± 1.59 in the 1000 mg *Euglena* intake group (*p* = 0.057 Steel's test). The amount of change in irritability from before Uchida–Kraepelin stress loading to 60 min after Uchida–Kraepelin stress loading in week 12 was 1.29 ± 2.02 in the placebo intake group and −0.54 ± 1.30 in the 1000 mg *Euglena* intake group, showing a significant easing of irritability caused by Uchida–Kraepelin stress (*p* = 0.008, Steel's test).

The amount of change in the level of subjective satisfaction with sleep from week 0 to 4 was -0.89 ± 1.44 in the placebo intake group and −2.61 ± 1.75 in the 3000 mg *Euglena* intake group, showing a significant increase in the level of subjective satisfaction with sleep (*p* = 0.007, Steel's test). The amount of change in the level of subjective satisfaction with sleep from week 0 to 12 was −0.65 ± 1.22 in the placebo intake group, −2.22 ± 3.08 in the 500 mg *Euglena* intake group, −2.69 ± 2.31 in the 1000 mg *Euglena* intake group, and −3.41 ± 2.26 in the 3000 mg *Euglena* intake group, showing a significant difference increase in the level of subjective satisfaction with sleep (*p* = 0.028, *p* = 0.012, *p* = 0.0006, respectively, Steel's test).
