*2.4. Animal Experiments*

The protocol for animal study was approved by the Department of Biofood Research, KNOTUS Life Science Co., Ltd. Animal Ethics Committee (17-KE-265). Male C57BL/ JJmsSlc mice, five weeks old (18–20 g), were purchased from Central Lab Animal Inc. (Seoul, Republic of Korea). The animals were housed in polycarbonate cages (less than five mice/cage) under controlled temperatures (23 ± 3 ◦C), relative humidity (55% ± 5%), and lighting conditions (lights on from 08:00 to 20:00 h), with food and water made available ad libitum. Mice were fed either a regular diet (ND group; lab rodent chow; Cargill-Agri Purina, Seongnam-Si, Republic of Korea) or a 60% HFD (HFD group; Saeronbio, Uiwang-si, Republic of Korea). On the 19th day of HFD feeding, the body weight of the HFD group was 10% higher than that of the ND group. At this time, the HFD group was divided into six additional groups: an HFD control group, a DKB-117-100-treated group (100 mg/kg/day), a DKB-117-200-treated group (200 mg/kg/day), a DKB-117-300-treated group (300 mg/kg/day), an orlistat-treated group (40 mg/kg/day), and a *Garcinia cambogia*–treated group (200 mg/kg/day), respectively. For eight weeks, the mice were daily treated with test article and HFD, with the exception of the ND group.

After eight weeks of treatment, the mice were not fed for 15 h and their body weight was measured. Then, the mice were anesthetized with ether. Blood was drawn from the postcaval vein for serum biochemical analysis. Extracted liver, abdominal fat, and inguinal fat tissue were weighed.
