*Article* **Anti-Obesity E**ff**ect of** *DKB-117* **through the Inhibition of Pancreatic Lipase and** <sup>α</sup>*-***Amylase Activity**

**Do Hoon Kim 1,**†**, Yu Hwa Park 1,**†**, Jung Suk Lee 2, Hyun Il Jeong 2, Kye Wan Lee 2 and Tong Ho Kang 1,\***


Received: 25 August 2020; Accepted: 2 October 2020; Published: 6 October 2020

**Abstract:** This study sought to evaluate the effects of *Phaseolus multiflorus* var. albus Bailey extract (PM extract) and *Pleurotus eryngii* var. ferulae extract (PF extract) on the inhibition of digestive enzymes and to confirm the anti-obesity effect of DKB-117 (a mixture of PM extract and PF extract) in digestive enzyme inhibition in a mouse model of obesity induced by a high-fat diet. In in vitro studies, PM extract and PF extract have increased dose-dependent inhibitory activity on <sup>α</sup>*-*amylase (Inhibitory concentration (IC50 value: 6.13 mg/mL)) and pancreatic lipase (IC50 value; 1.68 mg/mL), respectively. High-fat diet–induced obese mice were orally administered DKB-117 extracts at concentrations of 100, 200, and 300 mg/kg/day, while a positive control group was given orlistat (pancreatic lipase inhibitor) and *Garcinia cambogia* (inhibiting the enzymes needed to synthesize carbohydrates into fat) at concentrations of 40 and 200 mg/kg/day, respectively, for eight weeks. As a result, body weight, fat mass (total fat mass, abdominal fat, and subcutaneous fat) detected with microcomputed tomography, fat mass (abdominal fat and inguinal fat) after an autopsy, and liver triglyceride levels were decreased significantly in the DKB-117 (300 mg/kg/day) group compared to those in the HFD control group. Additionally, we obtained results indicating that the presence of carbohydrates was found more in the DKB-117-300 (300 mg/kg/day) group than in the HFD control group. These data clearly show that DKB-117 extracts are expected to have an anti-obesity effect through a complex mechanism that promotes carbohydrate release through the inhibition of carbohydrate-degrading enzymes while blocking lipid absorption through lipase inhibition.

**Keywords:** *Phaseolus multiflorus* var. albus Bailey (PM); *Pleurotus eryngii* var. ferulae (PF); anti-obesity; pancreatic lipase; α-amylase
