**E**ff**ects of** *Euglena gracilis* **Intake on Mood and Autonomic Activity under Mental Workload, and Subjective Sleep Quality: A Randomized, Double-Blind, Placebo-Controlled Trial**

#### **Ayaka Nakashima 1,\*, Kosuke Yasuda 1, Ako Murata 1, Kengo Suzuki 1 and Naoki Miura 2**


Received: 23 August 2020; Accepted: 20 October 2020; Published: 23 October 2020

**Abstract:** While the human body maintains homeostasis by altering the balance in the autonomic nervous, endocrine, and immune systems, a prolonged imbalance in these systems can result in physical and mental symptoms, including a decline in sleep quality and work efficiency. *Euglena gracilis* (*Euglena*) is a single-celled microalga with the properties of both plants and animals and contains abundant nutrients, such as vitamins, minerals, amino acids, and fatty acids, which have various beneficial health effects. This study evaluated the effects of *Euglena* intake on the mood states and stress coping under mental workload tasks, and subjective sleep quality. We assigned men and women aged 20 to 64 years to *Euglena* and placebo intake groups, and measured indices related to the autonomic nervous system, psychological states, and sleep quality together with the application of workload stress before food intake, and 4, 8, and 12 weeks after commencing intake. *Euglena* intake regulated the autonomic nervous system under a workload and improved psychological parameters and sleep conditions. These results indicate that the consumption of *Euglena* may regulate the balance of the autonomic nervous system during stress and may have a favorable effect on psychological status and sleep quality.

**Keywords:** *Euglena gracilis*; paramylon; autonomic nervous system
