**4. Conclusions**

The current research aims to isolate and identify regional bacteria generating symbiotic α-amylase enzymes in the intestines of short and talal fishes from Qeshm Island waters. In this study, the symbiotic bacteria *Bacillus* sp. HR13 and *Bacillus* sp. HR16 found in the intestines of *Sillago sihamas*, and *Rastrelliger Kanagurta* had the highest α-amylase enzyme activity at 60 ◦C. This implies they could be great choices for purification, mass production, and commercialization for use in the food and detergent industries. In general, the findings of this study show that the Persian Gulf is a rich source of bacterial strains that produce the widely used amylase enzyme, and the fish species *Sillago sihamas* and *Rastrelliger Kanagurta*, two of the most abundant species in the Persian Gulf, are rich in bacteria that adapt to the unique conditions of this region.

**Author Contributions:** Conceptualization and research design, A.H.; experimental, M.R.E.; data analysis, M.R.E. and A.H.; manuscript—writing, M.R.E.; manuscript—editing, A.H. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research received no external funding.

**Data Availability Statement:** All data were included in the manuscript.

**Acknowledgments:** The authors express their gratitude to the research council of the University of Hormozgan for financial support during the course of this project.

**Conflicts of Interest:** The authors declare no conflict of interest.
