*3.3. Cd2+ Resistance and Growth Curve of ST11*

The resistance of ST11 to Cd2+ was investigated. The Cd2+ concentrations ranged from 0 to 1.5 mmol/L for ST11. The cells were cultured with an LB medium without Cd2+ at 30 ◦C at 150 rpm in darkness for 24 h. After the cells were washed three times and resuspended with Milli-Q deionized water, 1 mL of cell culture (OD<sup>600</sup> = 1.0) was transferred into 150 mL sterilized flasks containing 30 mL of LB medium with various Cd2+ concentrations. The flasks were incubated at 150 rpm in darkness at 30 ◦C for 48 h. Cell growth was recorded by measuring the OD<sup>600</sup> of the medium. A survival curve was generated using the growth changes in cultures supplemented with various concentrations of Cd2+ compared with no-Cd2+-treated control samples. The regular interval for the biodegradation experiment was 48 h.
