*2.5. Measurement of Particle Size*

The distribution of particle sizes for hydrolysates and meals was determined using a laser diffraction system (Mastersizer 3000, Malvern Instrument Ltd., Worcestershire, UK) on hydrolysate and meal dispersions prepared by mixing 0.5 g sample (insect meals and hydrolysates) with 40 mL of McIlvaine buffer (pH 4.0, 5.5 and 7.0) for 12 h at 4 ◦C. The refraction index of sample dispersion corresponding to the protein refraction index was set to 1.45. The dispersant phase was deionized water and this refraction index was set to 1.33. The dispersion sample was added until an obscuration of approximately 10% was obtained. The particle size distribution was expressed as D3,2 (µm), the sautermean diameter. All conditions were conducted in duplicate.
