*3.5. Minerals Content*

The concentrations of the minerals Ca, Cu, Fe, K, Mg, Mn, Na, and Zn were determined using flame atomic absorption spectroscopy (F-AAS; SpectrAA-800, Varian, Palo Alto, CA, USA) preceded by microwave mineralization with nitric acid [92]. The recommendations for Ca, Cu, Fe, Mg, Mn, and Zn were established at the level of Nutrient Reference Value (NRV) [93]. The contents of minerals were expressed in g/100 g of the sample.
