2.1.2. Chemicals

Unless specified, all chemicals used for analytical purposes were analytical grade. Alcalase® (protease from Bacillus licheniformis), β-mercaptoethanol, D-L-Leucine, sodium tetraborate and o-phtaldialdehyde (OPA reagent) were purchased from Sigma Aldrich (St Louis, Missouri, MO, USA). Hexane, hydrochloric acid, methanol and sodium hydroxide were purchased from Fisher Scientific (Ottawa, ON, Canada). Citric Acid monohydrate, hydrochloric acid (36.5–38% *v*/*v*), NaCl, sodium hydroxide pellets and sodium phosphate dibasic were purchased from VWR International (Mississauga, ON, Canada). Sodium dodecyl sulfate (SDS) was purchased from Bio Basic (Markham, ON, Canada). Food grade canola oil was purchased from a local grocery store in Quebec City.
