*3.5. In Vitro Cytotoxicity Assay*

The human colorectal adenocarcinoma cell line HT-29 (Cat. no: 85061109), human gastric carcinoma Hs 746T cell line (ATCC® HTB-135™), human colon cancer Caco-2 cell line (ATCC® HTB-37™), and human normal colon mucosa CCD 841 CoN cell line (ATCC® CRL-1790™) were used in this study and cultured according to the method that was previously described in detail in this report [23]. The cell viability and metabolic activity were determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay [78]. The first cytotoxic dose (IC10), the median effective concentration (IC50), and the lethal dose (IC90) were calculated on the basis of the MTT results.
