*3.4. Basic Chemical Composition*

The moisture and ash content were measured by the constant mass method [38]. The total protein content was calculated from the amount of nitrogen by a 5.95 factor, evaluated according to the Kjeldahl method, using a Büchi Distillation Unit K-355 (Athens, Greece) (https://www.fao.org/3/y5022e/y5022e03.htm#bm3.1 (accessed on 20 April 2022)). Fat content was determined according to the standard method [38].
