*3.9. Effect on Intestinal Microflora*

To control the influence of the conditions prevailing in the gastrointestinal tract on the growth of microorganisms, control inoculations were made after 2 h from the moment (pH 7.4, small intestine) of introducing the microorganisms into the environment and at the moment of the termination of the digestion process (after 21 h). The intestinal microflora isolated from the faecalis of a mature person was introduced into the experimental model. The determined groups of microorganisms included: *Entrobacteriaceae* (MacConkey selective medium—Sigma Aldrich, Saint Louis, MO, USA), *Lactobacillus* (MRS agar medium—Sigma Aldrich, Saint Louis, MO, USA), *Enteroccocus* (substrate—agar with kanamycin, esculin, and sodium azide), and *Bifidobacterium* (Garche medium—Sigma Aldrich, Saint Louis, MO, USA). Inoculated media were incubated in anaerobic conditions depending on the determined group of microorganisms for the period of 48 to 72 h at 37 ◦C [27]. The number of viable bacterial cells was determined using Koch's plate method.
