*3.2. Chemical Analysis*

The Kjeldahl method was used to determine the total nitrogen content, which was then used to calculated the protein content using a nitrogen-to-protein conversion factor of 6.25 [70]. The international standard method ISO 763 [71] was used to measure the total ash content.

The concentrations of minerals Ca, Cu, Fe, K, Mg, Mn, Na, and Zn were determined using flame atomic absorption spectroscopy (FAAS) (SpectrAA-800, Varian, Palo Alto, CA, USA) that was preceded by microwave mineralization with nitric acid [72]. The recommendations for Ca, Cu, Fe, Mg, Mn, and Zn were established at the level of the Nutrient Reference Value (NRV) [73]. The contents of the minerals were expressed in g/100 g of the dry mass of the sample.
