*3.8. Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis (SDS–PAGE)*

SDS–PAGE analysis was performed according to the method of Laemmli [41]. The protein samples were diluted (10 mg/mL) with the buffer containing SDS and β-mercaptoethanol as the reducing reagents and were denatured. Then, the samples were loaded (10 µL) onto gel slabs (12%). At the end of the analysis, the gel slabs were stained with Coomassie Brilliant G-250 dye. Protein molecular weights were analyzed using the Infinity Capt (BioRad) program for electropherogram analysis.
