*2.4. Protein Solubility*

Suspensions of TMI, TMD, HDSP, and NDSP fractions (1% *w/v*) were prepared in Milli-Q water. The pH of suspensions was adjusted to 5, 7 or 9 with 1 M NaOH or 1 M HCl. The suspensions were centrifuged at 20,000× *g* for 30 min at 20 ◦C and filtered through a Whatman® Grade 1 filter paper. The filtrates were freeze-dried and the recovered fractions were analyzed for their protein content by the Dumas method with a 4.76 and 5.60 nitrogento-protein conversion factor, respectively for the meal larvae (TMI and TMD) and for the protein extracts (HDSP and NDSP) as described previously [26]. The protein solubility was calculated using Equation (1) [33]:

$$\text{Solubility}(\%) = \frac{\text{protein content in supernantant}}{\text{total protein content in sample}} \times 100\tag{1}$$
