*3.7. Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis (SDS-PAGE)*

SDS-PAGE analysis was performed according to the method of Laemmli [69]. The protein samples were diluted (10 mg/mL) with the buffer containing SDS and β-mercaptoeth anol as reducing reagents and denatured. Then, the samples were loaded (10 µL) onto

gel slabs (12%). At the end of analysis, gel slabs were stained with Coomassie Brilliant G-250 dye. The molecular masses of analysed proteins were estimated from the response curve of the mobility of molecular weight standards versus logMW.
