2.2.1. Lipid Extraction

Half of the TMI meal from each insect batch was stored at −20 ◦C while the other half was defatted using the Soxhlet method as described by Tzompa-Sosa et al. (2014) [23]. Briefly, 10 g of TMI were weighed into a cellulose cartridge (Fisher Scientific catalog number 12-101-100) and the lipids were extracted with hexane for 6 h. The defatted *T. molitor* meal (TMD) was recovered, vacuum-dried in an oven at 50 ◦C for 5 h and stored at −20 ◦C before further analysis and processing.
