4.2.2. Evaluation of Antifungal Activity

Antifungal activity was evaluated against *Aspergillus flavus* using disc diffusion method. Vogel's media was used for fungal culture growth. The selected polyurethane polymer samples were punched in disc of 5 mm diameter and applied over the place incubated at 37 ◦C overnight in incubator. Antifungal activity of 6 polyurethane samples with a variable content of Titanium dioxide and 1% xanthan gum was evaluated. The observed antifungal activity is shown in Figure 5. A clear zone of inhibition was observed on the plates of XTPU-5 and XTP-6; however, other polymer samples inhibited fungal growth at the area under the sample disc. The results exhibited that antifungal or biodegradability of polymer depends upon the content of TiO2 used. We may increase the quality by changing the composition. Moreover, the zone of inhibition can be increased by increasing the weight % age of TiO2.

**Figure 4.** Antibacterial assay of samples against *E. coli* and *Macrococcus*.

**Figure 5.** Antifungal assay of polyurethane samples against *Aspergillus flavus*.
