*4.2. Identification of the Best Bacterial Isolates Based on Molecular Method*

Isolates No. A6, P3 and W8 were identified according to the Polymerase Chain Reaction (PCR) method. The sequence of the A6 isolate was accessed using the accession number and it was found to belong to the *Bacillus paramycoides* strain MCCC 1A04098 (Figure 1A). Although several reports have produced PHB from *Bacillus* sp., such as *B. amyloliquefaciens*, *B. aryabhattai*, *B. brevis*, *B. cereus*, *B. circulans*, *B. coagulans*, *B. firmus*, *B. laterosporus*, *B. licheniformis*, *B. macerans*, *B. megaterium*, *B. sphaericus*, *B. subtilis*, and *B. thuringiensis* [1,14,43], and a few researchers [44–48] have used *B. mycoides* for producing PHB, to the best of our knowledge only this study has produced PHB from *B. paramycoides*.

In addition, the isolate No. P3 was identified as *Azotobacter salinestris* strain NBRC 102611 (Figure 1B). Three species of the genus *Azotobacter* sp. have commonly appeared in the literature on the production of PHB, including *A. chroococcum* [19,28,41,42,49], *A. vinelandii* [50–53], and *A. beijerinckii* [54–56]; however, only one used *A. salinestris* for PHB production [57] before this study. Isolate No. W8 was identified as the *Brevundimonas naejangsanensis* strain BIO-TAS2-2 (Figure 1C). Only one study has used *Brevundimonas naejangsanensis* for PHB production [58] prior to the current study. This study is also the first to report the newly isolated bacterial strain designated as *Bacillus paramycoides* strain MCCC 1A04098 as potential source for PHB production.
