*3.4. X-ray Diffraction Analysis*

Figure 11a–c shows the X-ray diffraction patterns of the bio-composite samples with different percentages of fenugreek, where intensity is shown vertically and the 2θ region is shown horizontally. The bio-composite sample with 5% fenugreek shows only two distinct peaks. Small peaks in the region of 2θ are represented in the bio-composite by the XRD pattern. Sharp peaks confirm the crystalline nature of the cellulose [43]. The peak at 2θ = 20◦ is attributed to the parallel-chain-segment aggregate region, which confirms the bio-composite semi-crystalline nature [44]. The region of 2θ = 28◦ also relates to the occurred disturbance in the cellulose at the time of the acetylation process, which can cause breakage in the micro-fibrillar structure of the cellulose [45,46]. However, the bio-composite samples with 10% and 15% fenugreek show no peaks, confirming the amorphous structure of the synthesized bio-composite samples.
