*Article* **Eco–Friendly Peelable Active Nanocomposite Films Designed for Biological and Chemical Warfare Agents Decontamination**

**Gabriela Toader 1,† , Aurel Diacon 2,† , Traian Rotariu 1,\* , Mioara Alexandru 3,\*, Edina Rusen <sup>2</sup> , Raluca Elena Ginghină <sup>4</sup> , Florentina Alexe 4, Ramona Oncioiu 4, Florina Lucica Zorila <sup>3</sup> , Alice Podaru 1, Andreea Elena Moldovan 1, Daniela Pulpea 1, Ana Mihaela Gavrilă 5, Tanta Verona Iordache <sup>5</sup> and Raluca S, omoghi 5,6**


**Abstract:** In the context of imminent threats concerning biological and chemical warfare agents, the aim of this study was the development of a new method for biological and chemical decontamination, employing non-toxic, film-forming, water-based biodegradable solutions, using a nano sized reagent together with bentonite as trapping agents for the biological and chemical contaminants. Bentonite-supported nanoparticles of Cu, TiO2, and Ag were successfully synthesized and dispersed in a polyvinyl alcohol (**PVA**)/glycerol (**GLY**) aqueous solution. The decontamination effectiveness of the proposed solutions was evaluated by qualitative and quantitative analytical techniques on various micro-organisms, with sulfur mustard (**HD**) and dimethyl methylphosphonate (**DMMP**) as contaminants. The results indicate that the peelable active nanocomposite films can be successfully used on contaminated surfaces to neutralize and entrap the hazardous materials and their degradation products. Mechanical and thermal characterization of the polymeric films was also performed to validate the decontamination solution's potential as peelable-film generating materials. The removal efficacy from the contaminated surfaces for the tested micro-organisms varied between 93% and 97%, while for the chemical agent **HD**, the highest decontamination factor obtained was 90.89%. **DMMP** was almost completely removed from the contaminated surfaces, and a decontamination factor of 99.97% was obtained.

**Keywords:** eco-friendly; nanoparticles; chemical warfare agents; sulfur mustard (**HD**); dimethyl methylphosphonate (**DMMP**); biological agents; nanocomposite films; decontamination; antimicrobial materials; peeling; active coatings
