*4.8. Confirmation of Ampicillin*−*Resistant Gene Deletion in Z. mobilis ZM4*

A pair of primers was used to confirm the disruption of each AR gene in *Z. mobilis* transformants: the primers were used to amplify the region including upstream of the corresponding gene, targeting gene, and downstream of the corresponding gene (see primer pairs in the Supplementary Materials Table S5, e.g., 0103−out−F and 0103−out−R).
