*4.7. Ion Leakage Assay*

*Dar Gazi-wt* infected with *E. amylovora* were collected 24 h after the inoculation and cut at the basal side which was submerged in the media. Plants were washed in de-ionized water twice and placed in 25 mL of de-ionized water. Each time point had triplicate samples for infected and non-infected plants. Solution conductivity was measured using a handheld conductivity meter, Type RS 180-7127 (RS Components), at the indicated times after plant collection.
