*2.3. Sugar Content*

Sugar content varied with the species, being more than 40% greater in turnip greens (>1.3 mg·g <sup>−</sup><sup>1</sup> FW) than in amaranth (<0.7 mg·<sup>g</sup> <sup>−</sup><sup>1</sup> FW) (Table 3).


**Table 3.** Main effects of species (amaranth and turnip greens) and LED treatment (W = white, B = blue, R = red) on total sugars and nitrate content of microgreens.

Values (mean ± *se*) within each column, followed by the same letter, do not significantly differ at *p* ≤ 0.05 according to Tukey's test; ns = not significant; significant at *p* ≤ 0.001 (\*\*\*). Three biological replicates were used for the analysis (*n* = 3).

Light did not exert any clear effect on this trait (*L*, *p* ≥ 0.05), while the interactive effect with species was not significant (*S* × *L*, *p* ≥ 0.05). replicates were used for the analysis (*n* = 3).

Values (mean ± *se*) within each column, followed by the same letter, do not significantly differ at *p* ≤ 0.05 according to Tukey's test; ns = not significant; significant at *p* ≤ 0.001 (\*\*\*). Three biological

**Table 3.** Main effects of species (amaranth and turnip greens) and LED treatment (W = white, B =

Species (*S*) Amaranth 0.7 ± 0.0 b 1990.9 ± 140.3 <sup>a</sup>

**(mg·g<sup>−</sup>1 FW)** 

Turnip greens 1.3 ± 0.0 a 704.9 ± 48.0 <sup>b</sup>

 *S* \*\*\* \*\*\* *L* ns \*\*\* *S* × *L* Ns \*\*\*

W 1.0 ± 0.4 1137.1 ± 202.5 <sup>b</sup> B 1.0 ± 0.4 1247.1 ± 318.7 <sup>b</sup> R 0.9 ± 0.4 1659.5 ± 357.7 <sup>a</sup>

**Nitrate (mg·kg<sup>−</sup>1)** 

### *2.4. Nitrate Content 2.4. Nitrate Content*  The nitrate content of shoots greatly varied with the species (*S*, *p* ≤ 0.001) (Figure 4).

LED treatment (*L*)

*Significance*

The nitrate content of shoots greatly varied with the species (*S*, *p* ≤ 0.001) (Figure 4). Greater amounts were measured in amaranth, where nitrates peaked under red light (>2000 mg·kg−<sup>1</sup> ). Lower contents (1583 to 1946 mg·kg−<sup>1</sup> ) were measured in this species in W and B, showing no difference according to ANOVA. Unlike amaranth, very low nitrate contents (<705 mg·kg−<sup>1</sup> ) were detected in turnip greens, regardless of the light conditions of growth (*S* × *L*, *p* ≥ 0.05). Greater amounts were measured in amaranth, where nitrates peaked under red light (>2000 mg·kg**−**1). Lower contents (1583 to 1946 mg·kg**−**1) were measured in this species in W and B, showing no difference according to ANOVA. Unlike amaranth, very low nitrate contents (<705 mg·kg**−**1) were detected in turnip greens, regardless of the light conditions of growth (*S* × *L*, *p* ≥ 0.05).

*Plants* **2021**, *10*, x FOR PEER REVIEW 6 of 19

blue, R = red) on total sugars and nitrate content of microgreens.

 **Total Sugars** 

**Figure 4.** Interaction effect of *species* × *light treatment* (W = white, B = blue, R = red) on the nitrate content (ppm) of microgreens. Data are means ± standard error (*n* = 3). Three biological replicates were used for the analysis. Different letters indicate significance at *p* ≤ 0.05 according to Tukey's test. **Figure 4.** Interaction effect of *species* × *light treatment* (W = white, B = blue, R = red) on the nitrate content (ppm) of microgreens. Data are means ± standard error (*n* = 3). Three biological replicates were used for the analysis. Different letters indicate significance at *p* ≤ 0.05 according to Tukey's test.
