*4.10. Statistical Analysis*

The experiment was performed using a completely randomized design. Three biological replicates were used for the analysis. Data were subjected to two-way ANOVA, and differences among means were determined using Tukey's post hoc test (*p* ≤ 0.05) All statistical analyses were performed using CoStat release 6.311 (CoHort Software, Monterey, CA, USA). The principal component loading plot and scores of PCA were obtained using Minitab 16, LLC. The data presented in the figures are the means ± *se* (Graphpad 7.0).
