*5.8. Real-Time Quantitative PCR Verification*

The second fully expanded leaf was harvested from five seedlings growing in each light treatment and used to determine RNA abundance. All the leaves were labeled and frozen in liquid nitrogen immediately. RNA was extracted using the TRIzolTM Plus RNA Purification Kit (TaKaRa Biotechnology, Dalian, China). Reverse transcription and amplification of cDNA were performed using Super Script III First-Strand Synthesis Super Mix for qRT-PCR (Vazyme, Nanjing, China). Real-time quantitative PCR was conducted in Real-Time PCR System (CFX96, Bio-rad, USA), and 2−∆∆CT method was used for data analysis [66]. The actin was selected as the reference gene. All target genes and target genes primers are listed in Supporting Information Table S1.
