**2. Materials and Methods**

#### *2.1. Study Design*

An observational prospective longitudinal cohort study has been designed. All patients included in the study were informed and gave their consent.

This study has been carried out according to the ethical principles of the Declaration of Helsinki and has been approved by the Clinical Research Ethics Committee (CEIC) of the East Area of Valladolid with code PI 19-1517.

### *2.2. Population and Study Period*

The study has been carried out from the following two cohorts of postmenopausal women: one cohort was women with obesity defined as BMI > 30 kg/m2; the other cohort was women without obesity defined as BMI < 30 kg/m2. The patients belonged to the East and West health areas of Valladolid, Spain.

A total of 250 patients were included in the study, including 124 postmenopausal women without obesity and 126 postmenopausal women with obesity, from whom data on anthropometry, food intake, and biochemical parameters related to bone metabolism in the initial assessment were taken; we also took fracture data five years after the start of study (January 2014).

The selection was made based on the following inclusion criteria: being a postmenopausal woman and being under 65 years of age. Exclusion criteria were being older than 65 years; criteria of premature or early menopause; have severe chronic kidney or liver disease and have the following toxic habits: active alcoholism and/or drug abuse.
