*Article* **Inhibition Effect of** *Pseudomonas stutzeri* **on the Corrosion of X70 Pipeline Steel Caused by Sulfate-Reducing Bacteria**

**Lina Qiu 1,2, Dandan Zhao <sup>1</sup> , Shujia Zheng <sup>1</sup> , Aijun Gong 1,2,\* , Zhipeng Liu <sup>1</sup> , Yiran Su <sup>1</sup> and Ziyi Liu <sup>1</sup>**


**\*** Correspondence: gongaijun5661@ustb.edu.cn

**Abstract:** Microbiologically influenced corrosion (MIC) is a common phenomenon in water treatment, shipping, construction, marine and other industries. Sulfate-reducing bacteria (SRB) often lead to MIC. In this paper, a strain of *Pseudomonas stutzeri* (*P. stutzeri*) with the ability to inhibit SRB corrosion is isolated from the soil through enrichment culture. *P. stutzeri* is a short, rod-shaped, white and transparent colony with denitrification ability. Our 16SrDNA sequencing results verify the properties of *P. stutzeri* strains. The growth conditions of *P. stutzeri* bacteria and SRB are similar, and the optimal culture conditions are about 30 ◦C, pH 7, and the stable stage is reached in about seven days. The bacteria can coexist in the same growth environment. Using the weight loss method, electrochemical experiments and composition analysis techniques we found that *P. stutzeri* can inhibit the corrosion of X70 steel by SRB at 20~40 ◦C, pH 6~8. Furthermore, long-term tests at 3, 6 and 9 months reveal that *P. stutzeri* can effectively inhibit the corrosion of X70 steel caused by SRB.

**Keywords:** microbiologically influenced corrosion inhibition; sulfate-reducing bacteria; *Pseudomonas stutzeri*; X70 steel
