*2.5. UV-Vis Spectroscopy Measurements*

UV-Vis spectroscopy measurements were performed with the Infinite M200 Pro plate reader (Tecan Group, Männedorf, Switzerland). For determination of the encapsulation efficiency (EE) and the loading capacity (LC) of the BRP-187 in the PCL particles, lyophilized NPs were dissolved in DMSO, and the solutions were investigated in a flat-transparent 96-well quartz plate (Hellma, Jena, Germany) at λ = 316 nm with 3 × 3 multiple reads per well and a 2000 µm well border. A calibration curve of BRP-187 was obtained for each batch in the concentration range of 1.2 to 312.5 µg mL−<sup>1</sup> with R<sup>2</sup> = 0.9997. The LC was calculated as follows: LC = (mass of drug recovered)/(mass of particle recovered) x 100. The EE was calculated as follows: EE = LC found/(mass of drug used) × 100. The determination of PVA in the NPs (%, *w/w*) was performed according to the published protocol [23].
