*2.4. Protein Adsorption*

BFG, HSA, and LYS were selected for protein adsorption experiments to investigate the anti-protein adsorption properties of the films. The films were soaked in 1 mL protein solution (1 mg/mL) and incubated at 37 ◦C for 1 h, and then washed with PBS buffer solution (pH 7.4) and ultrapure water in order to remove the unabsorbed proteins on the surface. A total of 0.5 mL SDS (1% *w*/*v*) solution was used to elute the adsorbed proteins and incubated with films at 37 ◦C for 2 h. The amount of protein was calculated by measuring the absorbance of the eluate at 562 nm with a UV–Vis spectrophotometer (Thermo Fisher Evolution 220, Waltham, MA, USA) using the BCA assay.
