4.2.6. Self-Assembling Peptides

Human MSCs were encapsulated within a 3D culture and grown on top of 2D culture biomimetic self-assembling peptide (SAP) hydrogel containing 1 mg/mL RGDs**Appendix A** 

**Appendix A** 

Hwang et al.,

Zhou et al.,

Hwang et al.,

Zhou et al.,

Tsimbouri et

Murphy et

Tsimbouri et

Murphy et

2021 [149] hMSCs

2021 [149] hMSCs

2019 [148] hMSCs Three bilayers of colla-

2019 [148] hMSCs Three bilayers of colla-

al., 2017 [150] MSCs Collagen gel

al., 2012 [151] MSCs Colla-

al., 2012 [151] MSCs Colla-

al., 2017 [150] MSCs Collagen gel

Nano-particulate mineralized collagen glycosaminoglycan

Nano-particulate mineralized collagen glycosaminoglycan

gen/glycosaminoglycan

gen/glycosaminoglycan

stem/progenitor cells.

stem/progenitor cells.

functionalized peptide (KFE–RGD) at the shear moduli of 0.25, 1.25, 5, and 10 kPa. Changes in adipogenic and osteogenic gene expression were relatively modest with no visual signs of differentiation as mineral deposition. The cells maintained a fibroblast-like phenotype throughout the culture period. However, on introducing 1:1 mixed adipogenic/osteogenic induction medium, the stiff matrices of 10 kPa induced the most efficient osteogenesis, with alizarin red-stained calcium deposits [203].
