*2.2. Enzymatic and Mechanical Treatments*

The softwood pulp was beaten in a PFI mill at 4000 revolutions under a refining intensity of 3.33 N/mm (ISO 5264-2) followed by an enzymatic treatment. The enzymatic treatment was performed according to the method described by Morais et al. [12]. The treatment was carried out at a consistency of 4% at pH 7 at 40 ◦C with continuous mechanical agitation to ensure an efficient mass transfer for 60 min and 30 mg of enzyme/g of pulp.
