*2.2. Automated High-Throughput Nanoprecipitation*

Automated high-throughput nanoprecipitation was performed in a 96-well plate (Greiner Bio-One GmbH, Frickenhausen, Germany) utilizing a FasTrans liquid handling robot (Analytik Jena GmbH, Jena, Germany). Starting with a polymer stock solution of 10 mg mL−<sup>1</sup> in THF, a dilution series with varying concentrations (0.25, 0.5, 1, 2, 3, 4, 5, 6, 7, 8, 9 and 10 mg mL−<sup>1</sup> ) was prepared. The polymer solutions (40 µL) were then automatically pipetted into 200 µL of either purified water (GenPure ultrapure water system, Thermo Scientific, Waltham, MA, USA) or PVA surfactant-containing aqueous solutions with a concentration of 0.25%, 0.5% or 1.0% (*w/v*). The resulting NP dispersions were mixed by pipetting up and down three times and then left for two hours for solvent evaporation. Each formulation was prepared twice. After solvent evaporation, the samples were diluted with pure water (1:2 ratio for a polymer concentration up to 4 mg ml−<sup>1</sup> and 1:10 ratio for all NPs prepared with a polymer concentration above 4 mg mL−<sup>1</sup> ) and investigated via dynamic light scattering (as described in Section 2.4) [22].
