*3.4. Michaelis–Menten Kinetics*

The maximum response current (*Imax*) and the apparent Michaelis–Menten constant (K app <sup>m</sup> ) were used to analyze the relationship between chronoamperometric signals and enzymatic reaction. As shown in Figure 5b, when glucose concentration exceeds 6 mM, a response plateau was observed with the characteristic of Michaelis–Menten kinetic mechanism. From the calibration plot (Figure 5b dotted line), the current response showed hyperbolic dependence on glucose concentration and was in good agreement with Michaelis– Menten kinetics [45]. The kinetic parameters, the maximum current generated during the enzymatic reaction (*Imax*) and the apparent Michaelis constant (K app <sup>m</sup> ) are the corresponding *a* and *b* parameters of hyperbolic function *y* = *ax*/(*b* + *x*) [46]. The apparent Michaelis– Menten constant (K app <sup>m</sup> ) is an indication of enzymatic mimics–substrate kinetics. From the hyperbolic calibration plot (Figure 5b dotted line), the *Imax* and Michaelis constant K app <sup>m</sup> were 40.34 µA and 3.75 mM, respectively. The value of K app <sup>m</sup> is consistent with the reported value (K app <sup>m</sup> = 3.84 mM) for other GOx immobilized on the chitosan complex over triangular silver nanoprisms/platinum biosensor [47].
