*2.7. Nuclear Magnetic Resonance (NMR) Analysis*

The linkage between the fructosyl moieties of the biosynthesized fructans was characterized by 1H NMR and 1C NMR. About 35 mg of purified sample was dissolved in 500 μL deuterium oxide (D2O) by bathing at 60 ◦C. The 1H NMR and 1C NMR spectra were recorded using an AVANCE III 400 MHz NMR spectrometer (Brucker Co., Billerica, MA, USA) at 60 ◦C, which used acetone (1H = 2.225 ppm) and 1, 4-dioxan (13 C = 66.50 ppm) as internal reference standards.
