*2.1. Chemical and Standards*

Olive, sunflower, sesame, and coconut oils (Mercadona, Madrid, MA, ESP). Diesel, hexane, toluene, trypticase soya agar (TSA), dextrans standard, 1,1-diphenyl-2-picryl-

hydrazyl radical (DPPH), H2O2, salicylic acid, Dulbecco's modified Eagle's medium (DMEM), polyoxyethylene sorbitan monolaurate (Tween 20), polyoxyethylene glycol sorbitan monooleate (Tween 80), sodium dodecyl sulphate (SDS), 2-[4-(2,4,4-trimethylpentan-2 yl)phenoxy]ethanol (Triton X-100), pyrogallol, HCl, ascorbic acid (Vc), fetal bovine serum (FBS), L-glutamine, penicillin, streptomycin (Sigma-Aldrich, St. Louis, MO, USA). JetQuick kit (Genomed, Leesburg, VA, USA), Sephadex G-100 column (Aldrich Chemical Company, Inc., Milwaukee, WI, USA), trifluoroacetic acid (TFA)(Aldrich®® Schnelldorf, Germany), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTM) (GE Healthcare, Uppsala, Sweden).

#### *2.2. Isolation of Bacterial Strain and PCR Amplification*

Extremophilic bacterial strain RT7 was isolated from the sediments of the river source in Río Tinto (Huelva), Spain (37◦43 19 N 6◦33 03 W). First, 10 mL of NaCl 0.6 M was added to 1 g of sediment, and the mixture was serially diluted (10-fold). Aliquots of 100 μL were inoculated on trypticase soya agar (TSA) plates and stored overnight at 30 ◦C. The isolated strain was preserved at −80 ◦C in 30% glycerol.

For the identification of the strain, PCR amplification was conducted as described by Abrusci et al. [31]. Genomic DNA was extracted from bacterial cells using an UltraClean microbial DNA isolation kit. The purified genomic DNA was used as a template to amplify the 16S rRNA gene with PCR using primers 27F (5 -AGA GTT TGA TC (C/A) TGG CTC AG-3 ) and 1492R (5 -TAC GG(CT) TAC CTT GTTACG ACT T-3 ). PCR amplifications were carried out in a Thermal Cycler 2720 (Applied Biosystems). The following were performed: an initial denaturing step at 94 ◦C for 5 min, the completion of 30 cycles of 1 min at 94 ◦C, 1 min at 56 ◦C, and 3 min at 72 ◦C; and a final extension of 72 ◦C for 10 min [32]. Amplicons were purified using the JetQuick kit, and sequenced using the ABI PRISM Big Dye Terminator Cycle Sequencing Ready Reaction Kit (ABI) and an Applied Biosystem ABI 310 (PE Applied Biosystems, Foster City, CA, USA) automated sequencer [33]. The obtained sequences were compared to those in the GenBank database using the BLAST program (National Center for Biotechnology Information). The selected sequences were aligned with CLUSTAL X [34].
