2.7.2. Detection of Non-Cohesive Spaces within Regenerated Epidermises

For quantification of abnormally regenerated epidermis areas, semi-quantitative estimation of non-cohesive spaces was performed on 6 whole-length HES-stained sections for each xenograft. Digital images were converted into binary pictures in greyscale using Fiji software. After establishing a threshold based on tissue-free areas of slides, expanded intercellular spaces present within regenerated epidermises were converted into red pixels, which were automatically quantified using an in-house routine (imagery platform of the Genethon institute, Evry, France). Dot plots cumulated the analyses of multiple regions for each section (numbers are indicated in figure legends).
