*2.8. Nrf2 Activity*

Nuclear protein fractions of irradiated MCF-7 cells were isolated using NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher, MA, USA), according to the manufacturer's instructions. Protein concentrations were assessed using the Bradford reagent (Bio-Rad). Nrf2 Transcription Factor Assay (Colorimetric) was performed using 20 mg of nuclear proteins (ab207223, Abcam) to detect nuclear Nrf2 and antioxidant responsive element (ARE) sequence binding at OD 450 nm, following the manufacturer's instructions.
