*2.7. qRT-PCR*

Total RNA was extracted using TRIzol (Invitrogen). cDNA was synthesized using reverse transcription, followed by quantitative real-time PCR with SYBR Green Supermix (Life Technologies), using primers for Nrf2, Keap1, HO1, NQO1, SOX2, NANOG, KLF4 and GAPDH, which were used as the normalizing control. miR200a detection was carried out using the stem-loop method, as described previously [16]. The gene-specific primers used to perform real-time qRT-PCR analysis are listed in Table S1.
