*2.4. Immunological Characteristics and Viability of MSCs*

The MSCs derived from the placenta immunophenotype was determined by flow cytometry. The expression of surface markers was evaluated using fluorochrome-labeled antibodies against CD34, CD45, CD90, CD105, CD73, and HLA-DR (BD Biosciences and Becman Coulter, Brea, CA, USA) on a FACSCanto II flow cytometer (Becton Dickinson, Durham, CA, USA) in accordance with the manufacturer's instructions.

Cell viability was evaluated using a 7-ADD dye that penetrates the cell's cytoplasmic membrane and binds to its DNA. The number of CD45-negative/7-ADD-positive cells was determined on a FACS Canto II flow cytometer (Becton Dickinson) in accordance with the manufacturer's instructions.
