3.5.3. Anti-Phytopathogenic Activity

All compounds were tested against five plant pathogens (*Colletotrichum asianum*, *C. acutatum*, *Fusarium oxysporum*, *Pyricularia oryzae* and *Curvularia australiensis*) by disk method [40]. DMSO was used as a negative control, carbendazim as a positive control. The concentration values of all test groups, negative control and positive control were 1 mg/mL; the anti-phytopathogenic results were recorded on a vernier caliper.

#### 3.5.4. Inhibitory Activity against α-Glucosidase

The *α*-glucosidase inhibitory activity of the tested compounds was determined using the method in [17], with modifications for carrying it out in 96-well plates. The initial concentration of all test samples (including positive control and negative control) was 1 mg/mL, the optimized method was a mixture of 0.1 mM potassium phosphate buffer (pH = 6.8, 0.5 mL) and 10 mg/L *α*-glucosidase (100 μL), the testing sample (0.5 mL) was incubated at 37 ◦C for 5 min, and the 2.5 mM (4-nitrophenyl-*β*-*D*-glucopyranoside) PNPG (0.5 mL) was added, followed by mixing. The reaction was carried out at 37 ◦C for 15 min and then stopped by adding 0.2 M solution of Na2CO3 (0.75 mL). The inhibitory activity against α-Glucosidase was evaluated by a full wavelength multifunctional microplate reader measurement at 405 nm. Finally, inhibition rate = [(Acontrol − Acompound)/Acontrol] × 100%. The SPSS software was used to calculate the IC50 value. DMSO was used as the negative control and acarbose was used as the positive control (IC50 = 0.5 mM).
