*Communication* **Sesquiterpenoids from the Mangrove-Derived** *Aspergillus ustus* **094102**

**Pengyan Gui 1,†, Jie Fan 1,2,†, Tonghan Zhu 1,3, Peng Fu 1,4, Kui Hong 5,\* and Weiming Zhu 1,4,\***

> 1 Key Laboratory of Marine Drugs, Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China; 11180811003@stu.ouc.edu.cn (P.G.); fj900622@163.com(J.F.);sdueduzth@126.com(T.Z.);fupeng@ouc.edu.cn(P.F.)


**Abstract:** Four new drimane sesquiterpenoids (**1**–**4**) and three known ones (**5**–**<sup>7</sup>**) were isolated from the fermentation broth of the mangrove-derived *Aspergillus ustus* 094102. Compound **5** was further resolved as four purified compounds **5a**–**5d**. By means of extensive spectroscopic and ECD analysis as well as the chemical transformation, their structures were identified as (2*R*,3*R*,5*S*,9*R*,10*S*)-2,3,9,11- tetrahydroxydrim-7-en-6-one (ustusol F, **1**), (2*R*,3*R*,5*R*,9*S*,10*R*)-2,3,11-trihydroxydrim-7-en-6-one (9- deoxyustusol F, **2**), (3*S*,5*R*,9*R*,10*R*)-3,11,12-trihydroxydrim-7-en-6-one (ustusol G, **3**), (5*S*,6*R*,9*S*,10*S*, 11*R*,2-*E*,4-*E*)-(11-dideoxy-11-hydroxystrobilactone A-6-yl)-5-carboxypenta-2,4-dienoate (ustusolate H, **4**), ((5*S*,6*R*,9*S*,10*S*)-strobilactone A-6-yl) (2*E*,4*E*)-6,7-dihydroxyocta-2,4-dienoate (ustusolate I, **5**), (2-*E*,4-*E*;6-,7--*erythro*)-ustusolate I (**5a**) and (2-*E*,4-*E*;*ent*-6-,7--*erythro*)-ustusolate I (**5b**), (2-*E*,4-*E*,6-*R*,7-*R*)- ustusolate I (**5c**) and (2-*E*,4-*E*,6-*S*,7-*S*)-ustusolate I (**5d**), (5*S*,6*R*,9*S*,10*S*,2-*E*,4-*E*)-(strobilactone A-6-yl)- 5-carboxypenta-2,4-dienoate (ustusolate J, **6**), and (2*S*,5*S*,9*R*,10*S*)-2,9,11-trihydroxydrim-7-en-6-one (ustusol B, **7**), respectively. Compound **5** showed antiproliferation against the human tumor cells CAL-62 and MG-63 with the IC50 values of 16.3 and 10.1 μM, respectively.

**Keywords:** drimane sesquiterpenoids; absolute configuration; antiproliferation; *Aspergillus ustus*; mangrove-derived fungus
