3.5.1. Antioxidant Activity

The antioxidant activity assay was based on the reported methods [15]. The assay was performed on a 96-well microplate, the reaction was initiated by adding 10 μL of sample solution to 200 μL of ABTS working solution. All test group gradients (including positive control) were of 2.0, 1.0, 0.5, 0.25 mg/mL, respectively. PBS buffer was used as the blank control, DMSO as the negative control, and trolox as the positive control (IC50 = 0.29 mM). The antioxidant effect was evaluated by a full wavelength multifunctional microplate reader measurement at 734 nm. The inhibition rate of each sample was calculated according to the following formula: inhibition rate = [(Ablank − Acompound)/Ablank] × 100%. Finally, the SPSS software was used to calculate the IC50 value.
