3.5.2. Antibacterial Activity

All compounds were determined against four pathogenic bacteria: *Staphylococcus aureus* (ATCC 25923), *Escherichia coli* (ATCC 25922), *S. epidermidis* (ATCC 17749) and *Pseudomonas aeruginosa* (ATCC 17749). The concentration value of the test group and positive control was 1mg/mL by the microplate assay method [28]. The antibacterial effect was evaluated by a full wavelength multifunctional microplate reader measurement at 630 nm; the broth medium containing pathogenic bacteria was used as the blank group and DMSO as the negative control, ciprofloxacin was used as the positive control. The positive control ciprofloxacin showed antibacterial activities against four pathogenic bacteria *S. aureus*, *E. coil*, *S. epidermidis* and *P. aeruginosa* with the MIC values of 0.097, 0.78, 0.195 and 0.78 μg/mL, respectively.
