*3.1. General Procedures*

The specific rotations were obtained on an ATR-W2 HHW5 digital Abbe refractometer (Shanghai Physico-optical Instrument Factory, Shanghai, China). The UV spectra were determined using a Shimadzu UV-2600 PC spectrophotometer (Shimadzu Corporation, Tokyo, Japan), while the ECD spectra were measured on a JASCO J-715 spectra polarimeter (Japan Spectroscopic, Tokyo, Japan). The 1H, 13C, and 2D NMR spectra were recorded on a Bruker AV 400 NMR spectrometer using TMS as an internal standard. High-resolution ESI-MS was performed on an LCMS-IT-TOF instrument (Shimadzu Corporation, Tokyo, Japan) using peak matching. TLC and column chromatography (CC) were carried out over silica gel (200–400 mesh, Qingdao Marine Chemical Inc., Qingdao, China) or Sephadex-LH-20 (18−110 μm, Merck, Darmstadt, Germany), respectively. HPLC analysis was measured on Wasters e2695 (Waters Corporation, Milford, MA, USA) using a C18 column (Waters, 5 μm, 10 × 150 mm). Semi-preparative HPLC was achieved on an Agilent Technologies 1200LC instrument with a C18 column (Agilent Technologies 10 mm × 250 mm). High-speed centrifugation was performed using a TGL-16B Anting centrifuge (Anting Scientific instrument Factory, Shanghai, China). The constant temperature water bath was in HH-2 thermostat water baths (Hervey Biotechnology Corporation, Jinan, China). Liquid fermentation was carried out in an ATL-03202 High-precision CNC shaking machine (Shanghai Kanxin Instrument and Equipment Corporation, Shanghai, China). The purity of the isolated

compounds was determined via high-performance liquid chromatography (HPLC), which was performed on an Agilent 1200 instrument and a reverse-phase column (4.6 × 150 mm, 5 μm). The UV wavelength for detection was 210 nm. All the crude extracts and compounds were eluted with a flow rate of 0.8 mL·min−<sup>1</sup> over a 50 min gradient (solvents: A, H2O; B, MeOH), as follows: 0–5min, 25% B; 5–15 min, 25–30% B; 15–30 min, 30–55% B; 30–40 min, 55–75% B; 40–50 min, 70–90% B (Figure S45).
