*3.3. Antibacterial Test*

Antibacterial properties of tissues sprayed with chitin were analysed through the enumeration of bacteria on the surface according to ISO/CD 8784-2 Standard. The obtained results (Table 3) showed a clear effect of chitin on bacteria proliferation that was almost halved at 1 wt% and was a fifth at 7.5 wt%. Although bacterial proliferation was not inhibited even at the highest concentration of chitin, its significant decrease can be considered an improvement in the capacity of the prepared tissue to limit bacteria growth.

**Table 3.** Results for bacteria enumeration on tissues treated with ChNFs (percentage of chitin was referred to the weight of raw tissue).

