*4.7. Supplementary Methods*

Additional Supporting Information can be found online in the Supplementary Materials tab for this article.

**Supplementary Materials:** Supplementary materials can be found at http://www.mdpi.com/1422-0067/21/11/ 4145/s1.

**Author Contributions:** Conceptualization, T.A., T.M. (Tomoyuki Mukai), and Y.M.; formal analysis, T.A., T.M. (Tomoyuki Mukai), and T.M. (Takafumi Mito); funding acquisition, T.M. (Tomoyuki Mukai) and Y.M.; investigation, T.M. (Tomoyuki Mukai); methodology, T.A. and T.M. (Tomoyuki Mukai); project administration, T.M. (Tomoyuki Mukai); software, T.A.; supervision, T.M. (Tomoyuki Mukai) and Y.M.; validation, T.A., T.M. (Tomoyuki Mukai), K.K., S.T., S.F., H.A.U., and Y.M.; writing—original draft, T.A., T.M. (Tomoyuki Mukai), and Y.M.; writing—review and editing, T.A., T.M. (Tomoyuki Mukai), T.M. (Takafumi Mito), K.K., S.T., S.F., H.A.U., and Y.M. All authors have read and agreed to the published version of the manuscript.

**Funding:** This work was supported by grants from JSPS KAKENHI (20K08814 and 17K09991 to Y.M.), Kawasaki Medical School (Research Project Grants; R01-060 and 30-051 to Y.M.), and UCB Japan (T.M., Tomoyuki Mukai).

**Acknowledgments:** We would like to thank M. Sato and H. Nagasu (Department of Nephrology and Hypertension, Kawasaki Medical School), Y. Jyu (Department of Health and Sports Sciences, Kawasaki University of Medical Welfare), and T. Sone (Department of Nuclear Medicine, Kawasaki Medical School) for technical assistance and critical suggestions; Y. Mino, M. Yoshimoto, K. Maitani, A. Kusumoto, H. Nakashima, N. Takemasa, and N. Obara for their technical assistance; and N. Nango (Ratoc System Engineering Co., Ltd.) for his kind instruction regarding the micro-CT analysis software. We are also indebted to N. Iwachidou and the staff at the Research Center of Kawasaki Medical School. We are especially grateful to David A. Fox (University of Michigan, Ann Arbor, MI) for critically reading the manuscript.

**Conflicts of Interest:** T.A., T.M. (Tomoyuki Mukai), T.M. (Takafumi Mito), K.K., S.T., S.F., and Y.M. received scholarship donations from Takeda Pharmaceutical Co., Astellas Pharma Inc., Merck & Co., Daiichi Sankyo Inc., Shionogi & Co., Chugai Pharmaceutical Co., and AYUMI Pharmaceutical Co. H.A.U. belongs to the Department of Chronic Kidney Disease and Cardiovascular Disease, which is endowed by Kawanishi Holdings, Chugai pharmaceutical Co., Boehringer Ingelheim GmbH, and Terumo Co. The funders had no role in the design of the study; the collection, analyses, or interpretation of data; the writing of the manuscript; or in the decision to publish the results.
