*4.6. Statistical Analysis*

All values are given as the mean ± standard error of the mean (SEM). A two-tailed unpaired Student's *t*-test was used to compare two groups, and a one-way analysis of variance (ANOVA) followed by Tukey's post-hoc test was used to compare three or more groups by using GraphPad Prism 5 (GraphPad Software, San Diego, CA, USA). *p* values lower than 0.05 were considered statistically significant.
