*4.3. Body Weight, Paw Volume, and Clinical Score*

Body weight, paw volume, and clinical score were measured on days 0, 3, 6, 9, and 12, and daily from then (Figure 2). Paw volume was measured with a water replacement plethysmometer (Unicom

Japan, Tokyo, Japan). The clinical score was defined as follows: score 0, normal paw; score 1, inflammation and swelling of one toe; score 2, inflammation and swelling of > 1 toe with inflammation and swelling of the entire paw or mild swelling of the entire paw; score 3, inflammation and swelling of the entire paw; score 4, severe inflammation and swelling of the entire paw or ankylosed paw [32]. Specimens with maximum paw volume < 2.5 mL were excluded because immunization of those rats is insufficient. Thus, two rats were excluded, one in the control group and another in the T group.
