*4.6. Transient Transfection of miR-941 Inhibitors*

Isolated CD14+ monocyte was cultured in a-MEM with 10% FBS and M-CSF for 72 h in 96-well plates on dentine slices. Cells were then transfected with 10 nM hsa-miR-941 hairpin inhibitor or 10 nM miRNA hairpin inhibitor as a negative control (Dharmacon, Lafayette, CO, USA) using lipofectamine 3000 for 6 h based on manufacturer's instructions (Invitrogen, Carlsbad, CA, USA). The sequences of miR-941 inhibitor and negative control miRNA are GUGGGCCGACACACGUGUACACG and UCACAACCUCCUAGAAAGAGUAGA. The transfection efficiency was measured by qRT-PCR.
