*4.5. Cell Adhesion Assay*

Cell adhesion assay was performed using a CytoSelect™ Cell Adhesion Assay Kit (Cell Biolabs, San Diego, CA, USA), which used a fibrinogen-coated plate, according to the manufacturer's protocol with modifications. U937 cells cocultured with 500 ng/mL PMA for 24 h (7.5 <sup>×</sup> 104 per well) were added and incubated with 500 ng/mL PMA at 37 ◦C for 24 h in a humidified atmosphere containing 5% CO2. Lysis buffer/CyQuant® GR dyes (200 μL) were added and incubated for 20 min at room temperature with shaking. Finally, the mixture was transferred to a 96-well plate and analyzed using an ELISA microplate reader (Anthos Zenyth 3100, Cambridge, UK) with two separate readings (480 and 520 nm).
