*2.13. Viability Assay*

*E. histolytica* trophozoite controls and EhTrxR overexpressing trophozoites (2.5 × 104) were cultivated or not in the presence of 2 μM AF for 24 h. The cells were harvested at 400× *g* for 5 min, stained with Propidium iodide (1 μg/mL), and analyzed by flow cytometry. Flow cytometry was performed using Cyan ADP (Agilent Dako, CA, USA) and data from 10,000 cells were collected for each condition.
