*2.8. Statistical Analysis*

The clinical, hematological, and biochemical parameter data obtained were analyzed using GraphPad Prism software (San Diego, CA, USA). In this process, a descriptive analysis of the variables was carried out, estimating the frequency and dispersion measures for the quantitative variables, and proportions for qualitative variables. The results were expressed as means ± standard error of the mean (S.E.M.). The normality of the continuous variables was evaluated using the Shapiro–Wilk test. The normality of the continuous variables was evaluated using the Shapiro–Wilk test. For comparison between infected groups (positive control and experimental groups) and negative control, the one-way ANOVA test was employed, followed by Dunnett's test; taking statistically significant differences to be *p* < 0.05. This statistical approach was applied for analysis of hematological (except polymorphonuclear (PMN) count cells), and biochemical results. For comparison between groups, the two-way ANOVA test was performed, with a post hoc analysis using Tukey, and taking significant differences to be *p* < 0.05. The results of the relative weight organ, ultrasound, and immunohistochemical assays were analyzed with a two-way ANOVA test, and a post hoc analysis using Tukey, taking significant differences to be *p* < 0.05.
