*2.10. Mitochondrial Membrane Potential*

Mitochondrial membrane potential was analyzed in the *T. cruzi* epimastigotes using the MitoProbe JC-1 assay kit (Molecular Probes; Thermo Fisher Scientific). Epimastigotes (1 × 107 cells/mL) were loaded with 10 <sup>μ</sup>M 5,5- ,6,6- -tetrachloro-1,1- 3,3- -tetraethylimidacarbocyanine iodide (JC-1) and incubated for 40 min at room temperature. The fluorescence intensity ratio of red (540 nm excitation and 590 nm emission) to green (490 nm excitation and 540 nm emission) was measured using a multi-well fluorescence reader [43].
