*2.4. Endocytosis Assay*

Epimastigotes were submitted to endocytosis with 30 μg/mL of transferrin-FITC, hemoglobin-FITC, or BSA-FITC in Roswell Park Memorial Institute (RPMI) medium for 30 min at 28 ◦C. The parasites were fixed with 4% (*v*/*v*) paraformaldehyde in phosphatebuffered saline (PBS, pH 7.2) for 1 h for flow cytometry and imaging using the fluorescence microscope AxioObserver (Zeiss, Oberkochen, Germany) after staining with DAPI.
