*2.2. Adaptation of E. histolytica Trophozoites to AF*

The concentration of AF in *E. histolytica* trophozoite culture was progressively increased from 0 to 2 μM over a period of one month.

#### *2.3. Growth Rate of WT Trophozoites and AFAT*

The growth rate of WT trophozoites or AFAT and their viability were measured according to a previously reported protocol [31].
