**5. Conclusions**

In conclusion, our data show that after detecting each other, neutrophils rapidly transfer MPO to the surface of the amoeba, and that its activity could be processing the ROS of the amoebae that are necessary for the optimal induction of NETosis. In the context of parasite-neutrophil interaction, this observation is very relevant, as many parasites can inhibit respiratory burst in neutrophils but NETosis is easily induced. In general, this contribution supports the notion that parasite-induced NETosis is a very complex

process, involving the rapid and active exchange of molecules between parasite and host cells.

**Supplementary Materials:** The following are available online at https://www.mdpi.com/article/ 10.3390/antiox10060974/s1, Figure S1: Pyrocatechol and luminol do not affect the viability of *E. histolytica* trophozoites, Figure S2: Catalase failed to reduce NET induced by *E. histolytica*, Figure S3: H2DCFDA detects hydrogen peroxide in trophozoites.

**Author Contributions:** Conceptualization, J.C.C., C.R. and C.D.-G.; methodology, C.D.-G., J.F.J.-R. and M.N.; software, C.D.-G.; validation, C.D.-G. and J.F.J.-R.; formal analysis, S.M.-C., J.P.L., J.C.C. and C.D.-G.; investigation, C.D.-G. and J.C.C.; resources, J.C.C.; data curation, J.C.C., S.M.-C. and J.P.L.; writing, C.D.-G. and J.C.C.; visualization, J.C.C. and C.R.; supervision, J.C.C. and C.R.; project administration, J.C.C.; funding acquisition, J.C.C. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research was funded by Consejo Nacional de Ciencia y Tecnología (CONACyT), grant numbers 284830 (to J.C.C.) and 254434 (to C.R.) and Programa de Apoyo a Proyectos de Investigación e Innovación Tecnológica (PAPIIT-UNAM), grant number IN208020 (to J.C.C.).

**Institutional Review Board Statement:** The study was conducted according to the guidelines of the Declaration of Helsinki, and approved by the Ethical Committee for Studies on Humans of the Instituto de Investigaciones Biomédicas, UNAM (Ethical approved number: FMED/CI/RGG/13 January 2008).

**Informed Consent Statement:** Informed consent was obtained from all subjects involved in the study.

**Data Availability Statement:** Data is contained within the article and supplementary material.

**Acknowledgments:** César Díaz-Godínez is a student of the Programa de Ciencias Bioquímicas, UNAM, and is a recipient of a scholarship from Consejo Nacional de Ciencia y Tecnología (CONA-CyT), Mexico (742064). César Díaz-Godínez thanks Alan Javier Montoya–Hernández for providing support during preparation of the manuscript.

**Conflicts of Interest:** The authors declare no conflict of interest.
