*3.3. Transcriptomics of AFAT*

We used RNA sequencing (RNA-seq) to examine the mechanism of adaptation to AF. Transcriptomics of WT trophozoites vs. AFAT was compared. Our comparisons revealed that adaptation to AF has a strong effect on the *E. histolytica* transcriptome, with more than 500 upregulated and downregulated genes (Table S1).
