*2.2. Neutrophil Isolation*

Neutrophils were obtained from peripheral blood of healthy volunteers in line with the approach of García–García et al. [42] using Ficoll-PaqueR gradient (GE Healthcare) and hypertonic shock to lyse erythrocytes. Cells were resuspended in PBS pH 7.4, counted in hemocytometer and reserved at 4 ◦C until use. This study was carried out in accordance with the recommendations and approval of the Ethical Committee for Studies on Humans of the Instituto de Investigaciones Biomédicas, UNAM (Ethical approved number: FMED/CI/RGG/ 013/01/2008). All subjects signed a written informed consent.
