**3. Results**

### *3.1. Generation of AFAT*

Resistance to 2 μM of AF in the parasite *T. gondi* can be generated by exposure of the parasite to the mutagenic compound *N*-Ethyl-*N*-nitrosourea [29]. To the best of our knowledge, the development by natural selection of parasites resistant to AF has never been attempted. To address this knowledge gap, we adapted *E. histolytica* trophozoites to AF by progressively increasing the drug concentration over a period of one month to 2 μM. To check whether adaptation to AF has an effect on the growth of the parasite, we compared the doubling time of AF-adapted trophozoites (AFAT) with that of wild type (WT) trophozoites. We found that the doubling time of AFAT (13 ± 0.32 h) was significantly higher than the doubling time of WT trophozoites (10.6 ± 0.24 h).
