*2.2. Transcriptomic and Proteomic Expression Analysis of FhSOD1 and FhSOD3*

Stage-specific *F. hepatica* transcriptome datasets, previously described by Cwiklinski et al. (2015; PRJEB6904) [5] were interrogated to determine the differential transcription of the *FhSOD1* and *FhSOD3* genes, represented as the number of transcripts per million (TPM). *F. hepatica* proteomic datasets were interrogated to determine the FhSOD1 and FhSOD3 protein abundance, represented by the exponentially modified protein abundance index (emPAI) within the somatic proteome and the secreted protein fraction (secretome/ES proteins), using Scaffold (version Scaffold\_5.1.2, Proteome Software, Portland, OR, USA). Analysis of the somatic proteome was carried out for the metacercariae, NEJ (3, 24, and 48 h post-excystment) and immature life cycle stages using the data reported by Cwiklinski et al. (2018, 2021) [6,7]. Secretome analysis was carried out on the 24 h NEJ, immature, and adult liver fluke secreted proteins using the data reported by Cwiklinski et al. (2018, 2021) [6,7] and Murphy et al. (2020).
