*2.4. Viability of AFAT Exposed to H2O2, Paraquat, MNZ or GSNO*

The viability of WT trophozoites and AFAT exposed to H2O2 (2.5 mM for 30 min), paraquat (2.5 mM for 24 h), MNZ (5 μM for 24 h), or GSNO (350 μM for 2 h) (Sigma-Aldrich, Jerusalem, Israel) was determined by the eosin dye exclusion method [31].

#### *2.5. Measurement of Cytopathic Activity*

Cytopathic activity was assayed against HeLa cells (a kind gift from T. Kleinberger, Faculty of Medicine, Technion) (using a previously described protocol [32].
