*3.6. Level of ROS in AFAT*

The lower quantity of OXs in AFAT compared to the quantity of OXs in acute AF trophozoites [28] suggests that AFAT are less exposed to ROS. Consequently, we measured the level of ROS with dichloro-fluorescein (H2DCDFC) in acute AF trophozoites and in AFAT. We observed that the ROS level in AFAT is significantly lower than that in acute AF trophozoites (Figure 3E).

#### *3.7. Comparison between Transcriptomics and Redoxomics of AFAT*

We found that only two genes upregulated in AFAT (Gal/GalNAc lectin Igl1 EHI\_006980 and SNF7 family protein EHI\_077530) have their product oxidized (Table S3). None of the genes downregulated in AFAT have their product oxidized (Table S3).

#### *3.8. Comparison between Transcriptomics of AFAT and Redoxomics of Acute AF Trophozoites*

We found that 77 genes upregulated in AFAT have their product oxidized in acute AF trophozoites [28] (Table S3). The most abundant OXs belong to metabolite interconversion enzyme (PC00262), protein-binding activity modulator (PC00095), protein modifying enzyme (PC00260), and cytoskeletal protein (PC00085) (Figure 4A). Of the upregulated genes in AFAT that have their product oxidized in acute AF trophozoites, genes that encode for dehydrogenase (PC00092), such as NAD(FAD)-dependent dehydrogenase (EHI\_099700); oxydoreductase (PC00176), such as Pyruvate:ferredoxin oxidoreductase (EHI\_051060); and metabolite interconversion enzymes (PC00262), such as isopentenyl phosphate kinase (EHI\_178490), are significantly enriched according to the PANTHER statistical overrepresentation test (Figure 4B).

**Figure 4.** *Cont*.

**Figure 4.** Comparison between transcriptomics of AFAT and redoxomics of acute AF trophozoites. (**A**) PANTHER sequence classification of genes upregulated in AFAT that have their product oxidized in acute AF trophozoites. (**B**) PANTHER statistical overrepresentation test of upregulated genes in AFAT that have their product oxidized in acute AF trophozoites.

> Eight genes that are downregulated in AFAT have their product oxidized in acute AF trophozoites [28] (Table S3). These OXs are the uncharacterized proteins (EHI\_008120, EHI\_065710, and EHI\_110780), Asparagine–tRNA ligase (EHI\_126920), Cytosolic Fe-S cluster assembly factor NUBP1 (EHI\_047750), ribonuclease (EHI\_156310), and Flavodoxinlike domain-containing protein (EHI\_096710).
