*2.3. GC-MS Analysis*

For chromatographic and spectrometric characterization, a GC7890 Gas Chromatograph (Agilent Technologies, AT, Palo Alto, CA, USA) was used, which has an MSD 5975C mass selective detector (AT, Palo Alto, CA, USA); an electron impact ionizer (EI, 70 eV) with an aras/splitless injector (1:30 aras radius); and an MS-ChemStation G1701-DA Data System, including a WILEY, NIST, and QUADLIB 2007 spectral library. The columns used were DB-5MS fused silica capillary (J&W Scientific, Folsom, CA, USA) and DB-WAX (J&W Scientific, Folsom, CA, USA). The data obtained were reconstructed by automatic scanning, and the relative concentration of each compound was obtained by means of an AT 7890 Gas Chromatograph coupled to a flame detection system (FID, 250 ◦C). Identification was based upon chromatographic criteria (most importantly retention time and linear retention indices and the use of standard compounds) and spectrometric criteria (interpretation of the mass spectrum compared with standard compounds and databases) [11].
