*2.3. Skin Prick Test*

SPT was performed on the forearms of all enrolled subjects to confirm the diagnosis of a suspected type I allergy and identify the sensitization type. We used specific inhalant allergen extracts (Gramineae grass pollen (Gramineae mix/Phleum Pratense/Cynodon Dactilon), ambrosia, mugwort, wall pellitory (Parietaria Judaica/Parietaria Officinalis), olive pollen (Olea Europea), cypress pollen (Cupressaceae), birch, cat, dog, house dust mite (Dermatophagoides farina/Dermatophagoides pteronissinus), molds (Alternaria

Alternata/Aspergillus/Cladosporium), a histamine positive control, and normal saline as a negative control. The test was interpreted after 15–20 min of application, with a positive result defined as a wheal ≥3 mm diameter.
