*2.6. Bronchoalveolar Lavage Cell Counting*

Bronchoalveolar lavage (BAL) cells were separated from BALF by centrifugation at 1000× *g* for 3 min. Then, the supernatants were collected for ELISAs, and the cell pellets were resuspended in PBS. Slides of BAL cells were processed using a CytoSpin (CytoSpin, Hanil Science, Gimpo, Republic of Korea) at 500 rpm for 5 min and stained by a Diff-Quik staining kit (Sysmex, Kobe, Japan).
