*2.7. Vesicular RNA Extraction*

RNA extraction was performed from 200 μL of EV solution using the miRNeasy kit (Qiagen GmbH, Hilden, Germany), according to the manufacturer's instructions. The RNA concentration was assessed using the Qubit™ microRNA Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). RNA size distribution and yield were analyzed using the Agilent 2100 Bioanalyzer with the Small RNA analysis kit (Agilent Technologies, Santa Clara, CA, USA).
