*2.4. Detection of Allergen Sensitization*

We used the automated microfluidic-based multiplexed immunoassay system (BioIC Allergen-Specific IgE Detection Kit; GENERAL MEDICAL CO., LTD, Taichung, Taiwan) to measure fungal allergen sIgE in sera. The levels equal to or greater than Class 1 (≥1 AU) were considered positive. The sIgE to fungi allergens, including *Aspergillus flavus, Aspergillus niger, Penicillium chrysogenum, Botrytis cinerea, Fusarium solani, Rhodotoeula, Trichophyton rubrum, Saccharomyces, Penicillium, Penicillium notatum, Cladosporium herbarum, Aspergillus fumigatus, Candida albicans,* and *Alternaria alternata* were detected.
