*2.6. Statistical Analysis*

All data are presented as mean ± standard error of the mean (SEM) of 2–4 independent experiments using GraphPad Prism 6.0 software (GraphPad, San Diego, CA, USA). Data analyses were performed using unpaired two-tailed Student's *t*-test while comparing NHBF and DHBF, one-way ANOVA followed by Tukey's multiple comparison tests or two-way ANOVA followed by Sidak's multiple comparison tests for statistical analysis of the data. A *p* value < 0.05 was considered statistically significant.
