*2.2. EV Isolation*

After thawing at room temperature, conditioned medium samples were sequentially centrifuged at 4 ◦C to remove cell debris and large vesicles (10 min at 500× *g*, 20 min at 2000× *g* and 30 min at 10,000× *g*). Next, the samples were passed through a 0.22 μm filter (Millex-GS Syringe filter unit, Merck KGaA, Darmstadt, Germany) and the EVs were isolated using the exoEasy kit (Qiagen GmbH, Hilden, Germany) from 4 mL of basolaterally and 600 μL of apically collected conditioned medium, according to the manufacturer's protocol [25].
