**2. Materials and Methods**

In the present study, sample preparation was performed at the Dr Ishrat-ul-Ebad Institute of Oral Health Sciences (Department of Prosthodontics); however, the microbiological testing along with the disinfection interventions were undertaken at the Pakistan Council of Scientific & Industrial Research Laboratories. (PCSIR). The study assessed the antibacterial disinfection of PMMA denture resin polymer using individual and combined use of distilled water (DW), microwave disinfection (MW) and denture cleaning agent (DC). Disinfection of PMMA polymer was assessed on bacteria cultured at 24, 36, 48 and 96 h and with disinfecting durations of 1 to 5 min.
