**5. Conclusions**

Combination of MW irradiation with DC (sodium perborate) showed higher disinfection percentage of bacterial species on PMMA polymer denture bases compared to MW and DC alone. PMMA disinfection using DC displayed lower antimicrobial disinfection percentage than the combined use of MW and DC as well as MW alone at 1 min or 2 min disinfection for *E. coli*, *S. aureus* and *S. mutans*.

**Author Contributions:** Conceptualization, Z.C., S.M.R.K., S.M., M.A. and Z.A.M.; methodology, K.A.A., S.A., M.A.A., F.U.R.Q. and F.V.; software, K.A.A., F.V., M.A. and T.A.; validation, Z.C., S.M.R.K., S.M. and M.A.; formal analysis, S.A., Z.A.M., F.U.R.Q., M.A.A. and F.V.; resources, F.U.R.Q., T.A. and F.V; data curation, S.A., Z.A.M., Z.C., F.U.R.Q. and M.A.; writing—original draft preparation, F.V., T.A. and Z.C.; writing—review and editing, F.U.R.Q., S.A., K.A.A. and M.A. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research was supported by Princess Nourah bint Abdulrahman University Researchers Supporting Project number (PNURSP2022R6), Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia.

**Institutional Review Board Statement:** The study protocol was reviewed by the ethics and review committee of Dr. Ishrat-ul-Ebad Institute of Oral Health Sciences, Dow University of Health Sciences.

**Informed Consent Statement:** Informed consent was obtained and patients informed of the risks and benefits of the use of extracted teeth in the experiment.

**Data Availability Statement:** The data are available on contact from the corresponding author.

**Conflicts of Interest:** The author declares no conflict of interest.

#### **References**

