*2.9. Growth Curve*

To confirm the responses of the S1fa-overexpressing yeast cells to salinity, we conducted the growth curve of the yeast cells under salt stresses. The yeast cells were incubated under 28 ◦C until OD600 reached 0.3, and then were treated with 1 M NaCl in liquid URA medium. The OD600 values were observed after 12, 14, 16, 18, 20, 22 and 24 h, respectively. The results indicate that *Bra034084* and *Bra029784* overexpressing yeast cells were highly sensitive to NaCl stresses (Figure 8). Under normal conditions, the growth rate of the S1fa expressing cells was the same as that of EV, whereas under NaCl stresses, the optical density was significantly decreased compared with that of EV. The growth rate of the *Bra003132* and *Bra006994* overexpressing yeast cells showed no difference compared with that of EV, but was significantly higher than those of the *Bra034084* and *Bra029784* overexpressing yeast cells (Figure 8). These findings suggest that salinity stresses had detrimental effects on the growth of the *Bra034084* and *Bra029784* overexpressing yeast cells.
