**3. Results**

#### *3.1. Overview of Sequencing Data-Quality Control*

In this experiment, the leaves and roots of the Control (CK), Low Fe (LF), Spermidine (Spd) and Low Fe + Spd (LFS) were sequenced, and each treatment was replicated three times. The results showed that 99.98% of the nucleotides in the transcriptome sequencing data reached Q20, and 97.25% of the nucleotides exceeded Q30 (Table 1).

**Table 1. Statistics of transcriptome sequencing data.** Sample, sample name; Raw Read, the number of reads in total; Valid Read, the number of valid reads after de-junctioning, de-low quality, etc.; Valid Ratio, the proportion of valid reads; Mapped Reads, the number of reads that can be compared to the genome; Unique Mapped Reads, can only uniquely match to one position in the genome; Q20%, the percentage of bases with Q20% quality value ≥ 20 (sequencing error rate less than 0.01); Q30%, the percentage of bases with Q30% quality value ≥ 30 (sequencing error rate less than 0.001).



**Table 1.** *Cont.*
