*2.10. Subcellular Localization of the S1Fa Genes*

Subcellular localization is considered a key parameter for transcription factor responses to abiotic stresses. However, no evidence was found to support the translocation of the S1fa genes into the nucleus under salt stresses.

To confirm the subcellular localization, the S1fa genes were transiently expressed in the fusion of GFP in yeast, and the fluorescence was observed using a confocal microscopy. Without salt stress, the S1fa genes were observed in the nucleus as dot-like structures as presented in Figure 9. When treated with salt, *Bra034084* and *Bra029784* were translocated into the cell wall.

**Figure 8.** Growth curves of the S1fa gene overexpressing yeast cells under 1 M NaCl stress. EV (empty vector (yeast WT)) and cells with the overexpression *of Bra003132, Bra006994, Bra034084* and *Bra029784* were grown at 28 ◦C. Cell density was monitored after 12, 14, 16, 18, 20, 22 and 24 h after the treatment. The error bar represents the deviation of three independent replications.

**Figure 9.** The subcellular localization of the S1fa genes and the empty vector (EV) tagged with GFP and transiently expressed in yeast cells treated with 1 M NaCl. The images were obtained from GFP, merged and bright channels. Scale bar: 10 μm.
