*Article* **Salt Stress Induces Changes in Physiological Characteristics, Bioactive Constituents, and Antioxidants in Kenaf (***Hibiscus cannabinus* **L.)**

**Ziggiju Mesenbet Birhanie 1,2,†, Dawei Yang 1,†, Mingbao Luan 1, Aiping Xiao 1, Liangliang Liu 1, Chao Zhang 1, Ashok Biswas 1, Susmita Dey 1, Yong Deng 1,\* and Defang Li 1,\***


**Abstract:** Salinity stress is a major environmental threat in agricultural systems. Kenaf is a promising crop for the future for cultivation in salinity-affected soils because of its high phytoremediation potential. The current study aimed to investigate the effects of salt stress using six different sodium chloride (NaCl) concentrations (0, 50, 100, 150, 200, and 250 mM) on the plant growth, physiological characteristics, bioactive constituents, and antioxidant capacity of *H. cannabinus*. The results indicated that the NaCl stress induced significant reductions in plant height and in the dry and fresh weights of the leaf tissue. In addition, the K, Ca, Mg, and P concentrations in this tissue also decreased under NaCl stress treatment conditions. In contrast, the NaCl stress led to the accumulation of hydrogen peroxide (H2O2), superoxide anion (O2 •−), malondialdehyde (MDA), proline, total soluble sugar, and total soluble protein. Under NaCl stress, the levels of antioxidants, including phenolics and flavonoids, also increased. The gas chromatography–mass spectrometry (GC-MS) results showed that the volatile compounds, including heptacosane, 1-octadecanesulphonyl chloride, and tetratetracontane, were induced under the NaCl stress treatment. Furthermore, the salt stress significantly improved the antioxidant capacity of the leaf extracts. These findings may provide insight into how *H. cannabinus* plants respond to salt stress and may help improve its medicinal value under salt stress.

**Keywords:** *Hibiscus cannabinus*; salt stress; physiological changes; bioactive constituents
