*2.6. Phenotypic Analysis of SlBAG9 Overexpression Plants under Osmotic Stress*

To analyze the phenotype of *SlBAG9*-overexpressing plants under osmotic stress (drought and salt), two developmental stages were used for processing. For seed germination assay, the seeds of *Arabidopsis thaliana* ecotype Columbia-0 (WT) and *SlBAG9* overexpression line 2–12 and 4–9 [22] were surface-sterilized and sowed on 1/2 MS plates supplemented with or without 200 and 300 mM mannitol (M1, M2), 175 and 200 mM NaCl (N1, N2), and 1.0 and 1.5 μM ABA (A1, A2), and placed under 4 ◦C for 3 days, then moved to a growth room under a 16 h-light/8 h-dark photoperiod in the greenhouse. The seed germination rate (root emergence) was evaluated every day, and the number of seedlings with significantly expanded and green cotyledons were also evaluated. For seedling growth assay, the seven-day-old seedlings were transferred to 1/2 MS medium supplemented with 0 or 300 mM mannitol, 175 mM NaCl, and 1.5 μM ABA and grown for different days. After 3 days, the rosette leaves were sampled for qRT-PCR and after 14 days the photographs were taken.
