*2.11. Responses of Cell Wall Biosynthesis Genes to NaCl Stresses*

In eukaryotic organisms, the biological integrity depends on the cell wall, which is considered an essential structure that not only maintains the morphology, but also participates in protecting the cell from environmental influences. Here, in this study, we investigated the expression levels of cell wall biosynthesis genes as presented in Figure 10. The results suggest that the S1fa genes *Bra034084* and *Bra029784* significantly enhanced the expression levels of cell wall biosynthesis genes compared with EV-Ck (no stress) and EV (with stress). The expression levels of cell wall biosynthesis genes, including *Ccw14p*, *Cha1p*, *Cwp2p*, *Sed1p*, *Rlm1p*, *Rom2p*, *Mkk1p*, *Hsp12p*, *Mkk2p*, *Sdp1p* and *YLR194c*, were significantly enhanced, while the expression levels of *Bck1p* and *Ptc1p* were downregulated under salt stresses in *Bra034084* and *Bra029784* overexpressing yeast cells. The expression of the *Pst1p* gene showed no significant difference. These findings suggest that *Bra034084* and *Bra029784* activate the signaling pathways regulating cell wall integrity by stimulating transcriptional and post-transcriptional genes in response to salt stresses.

**Figure 10.** Quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis was used to assess the expression levels of cell wall biosynthesis genes under NaCl stresses. The error bar represents the deviation of three independent replications. The letters above the error bar represent significant differences at *p* > 0.05.
