**3. Results**

#### *3.1. Qualitative and Quantitative Assessment of Isolated Rohitukine*

A quantity of 1.6 g of rohitukine was obtained after purification from 200 g shade-dried *D. gotadhora* leaves, i.e., 0.8% dry weight. After purification, the compound was subjected to a purity check by TLC, HPLC and LC–MS, in which the isolated compound was found to have 98% purity compared with the standard (Figure 1).

**Figure 1.** HPLC and MS chromatograms showing the purity of rohitukine isolated from leaves of *D. gotadhora.* (**a**) Chromatogram of standard rohitukine, 1 mg/mL. (**b**) Peak of rohitukine isolated from *D. gotadhora*. (**c**,**d**) Mass spectra showing the presence of a single peak of rohitukine (MW 305.3).
