*4.4. S1fa Gene Promoter Analysis and miRNA Prediction*

The promoter sequences of Chinese cabbage S1fa genes (2000 bp upstream) were obtained from Chinese cabbage genome database (http://brassicadb.cn (accessed on 22 March 2022)). The online tool PlantCARE (https://bioinformatics.psb.ugent.be/we btools/plantcare/html/ (accessed on 22 March 2022)) was used to analyze the cis-elements in the promoter sequence [17]. To predict the miRNAs, the coding sequence of the S1fa genes was submitted to the psRNATarget server (https://www.zhaolab.org/psRNATarget/ (accessed on 22 March 2022)) as the targeted candidate as described previously [32].
