*4.3. Verification of the PARPis and PARGi E*ff*ects on VERO Basal PAR Pool*

ICF using an anti-PAR antibody on untreated VERO cells indicated that 25 nM OLA had an effect that was very similar to 250 nM OLA, diminishing the endogenous PAR more than 100 nM EB or 5 mM 3AB. Conversely, the PARG inhibitor DEA induced PAR accumulation.
