3.2.1. Materials

The oligonucleotides were purchased from Biosset (Novosibirsk, Russia). The reagents, solvents and the basic components of buffers were purchased from Sigma (St. Louis, MO, USA), Acros organics (Waltham, MA, USA) or Promega (Madison, WI, USA). γ–[32P]-ATP and α–[32P]-ATP (with specific activities of 5000 and 3000 Ci/mmol, respectively) were from the Laboratory of Biotechnology (Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russia). T4 polynucleotide kinase was from Biosan (Novosibirsk, Russia). SUMO fusion expression vector pETHSUL (GenBank: EF205333.1) and pSUPER vector coding for the catalytic domain of the S. cerevisiae SUMO hydrolase dtUD1 were kindly provided by Dr. P. Loll (Drexel University, Philadelphia, USA). His6 and Strep-II double-tagged dtUD1 hydrolase was expressed and purified as described in ref. [81]. Human recombinant poly(ADP-ribose)-polymerase 1 (EC 2.4.2.30) was prepared as described previously [82]. Murine recombinant poly(ADP-ribose)-polymerase 2 (EC 2.4.2.30) was purified as described previously [83]. Plasmid encoding human PARP-3 was kindly provided by Dr. A. Ishchenko (Gustave Roussy, Université Paris-Saclay, France). Poly(ADP-ribose)-polymerase 3 (EC 2.4.2.30) was purified according to [6].
