*4.9. Sub-Cellular Fractionation*

For sub-cellular fractionation experiment cancer cells were lysed according to the protocol by Mladenov et al. [42]. Briefly, cells were collected, washed in PBS and Triton X-100-soluble fraction was extracted by using CSK-buffer. The pellet was washed with buffer A and then extracted with buffer A containing Benzonase® Nuclease (Sigma, St. Louis, MO, USA). The supernatant was considered as a DNase-soluble fraction. The pellet was washed and extracted with buffers containing increasing concentrations of ammonium sulfate. The remaining pellet was labeled as the nuclear matrix fraction.
