*2.8. Cytokine Quantification*

The cytokines GM-CSF, IL-12p70, and IL-10 were determined by enzyme-linked immunosorbent assay (ELISA), following the indications of the manufacturer (Diaclone, Besançon, France). Cytokine concentration was expressed in *p*g/mL, as calculated from the calibration curves from serial dilution of human recombinant standards in each assay.

#### *2.9. Statistical Analysis*

All values are expressed as arithmetic means ± standard deviations (SD). Data were evaluated with Graph Pad Prism Version 5.01 software (San Diego, CA, USA). The statistical significance of any difference in each parameter among the groups was evaluated by one-way analysis of variance following Tukey's multiple comparisons test as a post hoc test. The Pearson *r* value was used to analyze the statistical significance of correlation test. *p* values less than 0.05 were considered statistically significant.

#### **3. Results**
