**Abbreviations**


### **Appendix A**

**Figure A1.** Immunolocalisation of the JIM8 epitope (**A**–**F**) in cross-sections of *B. distachyon* leaves (21 ◦C), (**A**–**C**): through the major vascular bundle; (**D**–**F**): through the edge of the leaf blade. The white arrows point to the respective parts of the leaf. Abbreviations: ep—epidermis, FB—fluorescent brightener, ib—inner bundle sheath, me—mesophyll, ob—outer bundle sheath, ph—phloem, pr—prickle, sc—sclerenchyma, vb—vascular bundle, ve—vessels, xp—xylem parenchyma. The green colour shows epitope occurrence. Scale bars: 20 μm.

**Figure A2.** Immunolocalisation of the JIM13 epitope (**A**–**I**) in cross-sections of *B. distachyon* leaves (21 ◦C), (**A**–**C**): through the major vascular bundle; (**D**–**F**): through the edge of the leaf blade; (**G**–**I**): prickle. The white arrows point to the respective parts of the leaf. Abbreviations: bc—bulliform cells, ep—epidermis, FB—fluorescent brightener, ib—inner bundle sheath, me—mesophyll, ob—outer bundle sheath, ph—phloem, pr—prickle, sc—sclerenchyma, vb—vascular bundle, ve—vessels, xp—xylem parenchyma. The green colour shows epitope occurrence. Scale bars: 20 μm.

**Figure A3.** Immunolocalisation of the MAC207 epitope in cross-sections of *B. distachyon* leaves (21 ◦C), (**A**–**C**): through the major vascular bundle; (**D**–**F**): through the mesophyll and bulliform cells. Abbreviations: ep—epidermis, b—bulliform cells, FB—fluorescent brightener, ib—inner bundle sheath, me—mesophyll, ob—outer bundle sheath, ph—phloem, ve—vessels, xp—xylem parenchyma. The green colour shows epitope occurrence. Scale bars: 20 μm.

**Figure A4.** Immunolocalisation of the LM6 epitope (**A**–**C**) in cross-sections of *B. distachyon* leaves (21 ◦C), through the major vascular bundle. The white arrows point to the respective parts of the leaf. Abbreviations: ep—epidermis, FB—fluorescent brightener, ib—inner bundle sheath, me—mesophyll, o—outer bundle sheath, ph—phloem, sc—sclerenchyma ve—vessels, xp—xylem parenchyma. The green colour shows epitope occurrence. Scale bars: 20 μm.

**Figure A5.** Immunolocalisation of the JIM11 (**A**–**I**), JIM12 (**D**–**I**) and JIM20 (**J**–**O**) epitopes in cross-sections of *B. distachyon* leaves (21 ◦C), (**A**–**F**,**J**–**L**): through the edge of the leaf blade; (**G**–**I**,**M**–**O**): through the major vascular bundle. Abbreviations: bc—bulliform cells, ep—epidermis, FB—fluorescent brightener, ib—inner bundle sheath, me—mesophyll, ob—outer bundle sheath, p— phloem, pr—prickle, sc—sclerenchyma, vb—vascular bundle, ve—vessels, xp—xylem parenchyma. The green colour shows epitope occurrence. Scale bars: 20 μm.


**Table A1.** The oligonucleotide primers that were used for the RT-qPCR reaction with relevant descriptions of the genes.

#### **References**


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