**4. Conclusions**

The concordance between the proteomics results and the cellular lipidemic activity could imply that the Lingzhi protein hydrolysate-loaded nano-liposomes induced cellular lipolysis without affecting cell viability. The proteomic study also indicated that loaded liposomes exhibited lipid accumulation with the suppression of FAS and ELOV5. Finally, other proteins including TSSK5, SMU1, GRM7\_MOUSE, and KLC4, were identified in the loaded liposome treatment group, associated with various biological mechanisms beyond lipid metabolism. Therefore, the nano-liposomal hydrolysates might serve as novel functional ingredients in the treatment and prevention of obesity.

**Supplementary Materials:** The following are available online at https://www.mdpi.com/2304-815 8/10/9/2157/s1, Figure S1: DLS analysis showed size distribution, Figure S2: DLS analysis showed zeta-potential distribution, Figure S3: Intragroup variation analysis.

**Author Contributions:** Conceptualization, S.K. and Y.Y.; methodology, T.M., C.C. and P.S.; software, S.K. and Y.Y.; validation, K.C., Y.Y. and K.C.; formal analysis, S.K. and Y.Y.; investigation, P.P.; resources, W.V. and K.C.; data curation, C.C. and K.C.; writing—original draft preparation, S.K.; writing—review and editing, S.K. and Y.Y.; visualization, T.M.; supervision, P.S. and C.C.; project administration, K.C.; funding acquisition, K.C. All authors have read and agreed to the published version of the manuscript.

**Funding:** This study was supported by the National Research Council of Thailand (NRCT) under the Royal Golden Jubilee Ph.D. Program (Grant No. NRCT5-RGJ63002-042) and Kasetsart University Research and Development Institute, KURDI (FF (KU)25.64).

**Institutional Review Board Statement:** Not applicable.

**Informed Consent Statement:** Not applicable.

**Data Availability Statement:** Not applicable.

**Acknowledgments:** This research project was supported by National Research Council of Thailand (NRCT): NRCT5-RGJ63002-042. Additional support for core research facilities was provided by faculty of pharmacy, Chiang-Mai university.

**Conflicts of Interest:** The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.
