**4. Conclusions**

In the present study, it was demonstrated that it is possible to formulate sugar-free milk chocolate formulations with added ω-3 PUFAs and probiotics, showing adequate acceptability by consumers. One of the drawbacks of the formulations evaluated was the decrease in acceptability by consumers when FO was added as an ingredient. Therefore, further studies should consider using lower concentrations of FO, or adding the ω-3 PUFAs from other sources, such as microalgae. The results presented herein support the idea that chocolate could be used as a good delivery system of bioactive ingredients, and thus further

studies should evaluate the effect on these new chocolate formulations on the prevention of diseases through the evaluation of their efficacy by in vitro, in vivo and clinical studies.

**Supplementary Materials:** The following are available online at https://www.mdpi.com/2304-815 8/10/8/1866/s1, Table S1: Fatty acid profile (mg fatty acid per 100 g sample FW−1) of fish oil used as a source of ω-3 polyunsaturated fatty acids.

**Author Contributions:** Conceptualization, A.R.G.-F., N.E.O.-S., A.S. and D.A.J.-V.; methodology, A.R.G.-F., P.F.-B., N.E.O.-S., L.M.M.-O., A.S., E.P.-C., C.H.-B. and D.A.J.-V.; formal analysis, A.R.G.-F., P.F.-B., N.E.O.-S., L.M.M.-O. and E.P.-C.; investigation, A.R.G.-F., P.F.-B., N.E.O.-S., A.S., E.P.-C. and D.A.J.-V.; resources, A.S., E.P.-C., D.A.J.-V. and C.H.-B.; writing—original draft preparation, A.R.G.-F. and D.A.J.-V.; writing—review and editing, E.P.-C., A.S., D.A.J.-V. and C.H.-B.; supervision, D.A.J.-V. and A.S.; funding acquisition, D.A.J.-V., C.H.-B. and A.S. All authors have read and agreed to the published version of the manuscript.

**Funding:** This study was based upon research supported by Tecnologico de Monterrey—Bioprocess Research Group, NutriOmics Research Group, Translational Omics Research Group, and VitaWard Research Foundation. Authors A.R.G.-H., P.F.-B. and L.M.M.-O. also acknowledge their scholarships #966535, #923811, and #445941 respectively, from CONACYT.

**Institutional Review Board Statement:** The study was conducted according to the guidelines of the Declaration of Helsinki and was approved by the Institutional Ethics Committee for Sensory Evaluation Studies of the Department of Bioengineering, School of Engineering and Sciences of Tecnologico de Monterrey (Ethics ID: CSERMBIGDL-002, approved on 31 May 2019).

**Informed Consent Statement:** Informed consent was obtained from all subjects involved in the study.

**Data Availability Statement:** The data presented in this study are available on request from the corresponding author.

**Conflicts of Interest:** The authors declare no conflict of interest.
