*4.8. Statistical Analysis*

Data were analyzed with the R software (www.r-project.org) using the one-way analysis of variance (ANOVA) for significant difference. The error bars were calculated with data from three replicates. ANOVA results were considered significant at *p* < 0.05 and mean comparisons were done using the Tukey HSD test.

**Supplementary Materials:** Supplementary Materials can be found at http://www.mdpi.com/1422-0067/20/19/ 4857/s1. Table S1. Full list of the unigenes annotated in *Pittosporum tobira* leaf; Table S2. Full list of the genes expressed in *Pittosporum tobira* leaf and their FPKM values. T01-T03 represent the three replicates libraries of the cultivar "Green Pittosporum" and T04-T06 represent the three replicates libraries of the cultivar "Variegatum"; Table S3. The primer sequences of genes used for real time quantitative PCR; Figure S1. qRT-PCR results of 10 selected genes and correlation between transcriptome data and real time PCR results.

**Author Contributions:** Z.Z. project design and paper writing; Z.L. Data processing and analysis; H.S. experimental test and data analysis; M.C. experimental test and data collection; S.C. project design and management. All authors have read and approved the final version of this manuscript.

**Funding:** This research was funded by the Project of Henan Science and Technology Research Program (Agricultural Field), grant number: 162120110070.

**Conflicts of Interest:** The authors declare no conflict of interest.
