5.1.1. Stable Expression

The abovementioned approaches of vector delivery into plant cells, such as biolistic and *Agrobacterium*-mediated transformation, are used to deliver the CRISPR/Cas DNA, and by screening the mutants, the DNA is inserted into the target location in the plant genome and carry out the editing phenomena. Currently, there are numerous applications of this system in plant GE. In 2016, Gao and colleagues incorporated a fluorescent gene in the expression cassette of CRISPR/Cas9 [146]. Recently, an interesting strategy was developed in which *BARNASE* and *CMS* were used as suicide genes to kill embryos and pollens containing the transgene of T0 plant progeny [147].
