*4.5. Sequence Divergence Analysis*

After aligning all sequences using Mafft v7.313 [78], we located and counted the SNP sites in the plastid genomes using DnaSP v6.0 [79]. The nucleotide variability (Pi) and polymorphic sites (S) were evaluated using a sliding window analysis with a step size of 200 bp and window length of 600 bp.
