*4.10. Determination of Leaf Relative Water Content, Chlorophyll, and Proline*

A separate experiment was conducted to measure biochemical and physiological traits related to salinity tolerance. "YNU31-2-4", "Kaijin", and "Yukinko-mai" seedlings were cultured hydroponically [86]. Ten-day-old rice seedlings were subjected to 0 or 125 mM NaCl (pH 5.0). Samples were collected 10 days after salt was imposed.

The relative water content (RWC%) of control and salt-treated leaves was determined according to Sade et al. [87] as:
