%RWC = (fresh weight − dry weight)/(turgid weight − dry weight) × 100

Fully expanded leaves of plants cultured in the absence or presence of salt stress were harvested at the end of the light period, snap-frozen, and ground to a fine powder in liquid nitrogen with a pestle and mortar. Total chlorophyll content was determined according to Lichtenthaler [88]. Free proline content was measured by a colorimetric assay as described by Bates et al. [89].
