*4.2. Multiple Sequence Alignment and Phylogenetic Analysis*

Multiple sequence alignments of 50 grapevine B3 sequences, 91 Arabidopsis B3 sequences and 82 tomato B3 sequences were performed using ClustalX 2.1 with default parameters [67]. The corresponding phylogenetic tree was constructed using the neighbor-joining (NJ) method and MEGA 6.0 software, using 1000 bootstrap replicates with the following parameters; "*p*-distance", "Complete Deletion", and gap setting [34]. Phylogeny among grapevine B3 sequences shown in Figure 2A was determined using MEGA 6 software by the neighbor-joining method with 1000 iterations and sequence alignments were performed using the 'W' approach.
