**5. Recent Breakthroughs in CRISPR**/**Cas9-Mediated Genome Editing in Plants**

The GE tool of the CRISPR/Cas9 system has gained remarkable importance in agriculture; however, there are some drawbacks which limit its application in plant GE. The major concerns related to this technology are non-specific off-targets; HR inefficiency; PAM sequences constriction; cargo-vector inefficiency; and many others. With the advancement of plant biotechnology many unique innovative steps are regularly incorporated into the GE network to tackle these challenges.
