*2.6. Immunohistochemistry*

Selected sections (4 μm) from the liver were immunohistochemically labelled with a panel of antibodies. In all the cases, a polymer-based detection system (EnVision® System Labelled Polymer-HRP; Dako, Glostrup, Denmark) was employed, following the manufacturer instructions. Subsequently, immunolabelling was developed with a solution of 3.3´diaminobenzidine (DAB) or AP solution (Vector Laboratories, Burlingame, CA, USA). The slides were counterstained with Mayer's haematoxylin and mounted in hydrophobic medium (Table 1).

**Table 1.** Antibodies, specificity and immunohistochemical procedure used.

