**4. Conclusions**

Based on histopathological and immunohistochemical results of the current study, we sugges<sup>t</sup> that exclusive glial response, including proliferation and hypertrophy of astrocytes and microglia, is a crucial event in the chronic neuronal damage, observed in the selective superficial neocortical neuronal degeneration of Irish Wolfhound dogs, without effective involvement of the lymphocytic response.

**Author Contributions:** All authors have contributed equally, read and agreed to the final version of the manuscript. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research received no external funding.

**Institutional Review Board Statement:** Ethical review and approval were waived for this study, because the samples analyzed were obtained in a necropsy of a dog that was euthanized after decision of the owner.

**Informed Consent Statement:** Not applicable.

**Data Availability Statement:** Not applicable.

**Acknowledgments:** The authors thank Tamara Rivero Balsera for the technical assistance and Ronaldo C. da Costa (OSU) for the critical review of the manuscript.

**Conflicts of Interest:** The authors declare no conflict of interest.
