*Article* **Determination of the Elements Composition in** *Sempervivum tectorum* **L. from Bulgaria**

**Galia Gentscheva 1,2, Irina Karadjova 3, Poli Radusheva 4, Stefka Minkova 4, Krastena Nikolova 4,\*, Yoana Sotirova 5, Ina Yotkovska 1 and Velichka Andonova 5,\***


**Abstract:** *Sempervivum tectorum* L. is an evergreen plant with fleshy blue-green leaves forming a rosette. The plant is well-known in alternative medicine and has been used for thousands of years. Traditionally for medicinal purposes, the plant is used as a juice obtained by simple squeezing of fresh plants leaves. The total content of Ca, K, Na, Mg, Mn, Fe, Zn, Cu, Co, Al, V, Cr, Ni, Mo, Ba, Pb, Cd, Hg, As, and Tl in plant leaves of *Sempervivum tectorum* L. sampled from different habitats in Bulgaria was determined after microwave digestion and measurements by inductively coupled plasma mass spectrometry and flame atomic absorption spectrometry. Furthermore, the bioavailable fraction of essential elements Ca, Mg, Fe, Mn, and Zn was defined after extraction with a hydrochloric acid solution, mimicking stomach juice. The total element content showed a high bioavailability of essential human health elements, such as Ca, Mg, Fe, and Zn. Additionally, essential and toxic elements concentrations were quantified in a fresh juice, obtained by squeezing from plant leaves, as most frequently used in folk medicine. The results obtained demonstrated high concentrations of K, Mg, Ca, Zn, and Cu, which could be accepted as an explanation and a further confirmation of the anti-inflammatory action of this plant.

**Keywords:** *Sempervivum tectorum* L.; mineral content; bioavailable fraction; heavy metals
