*2.3. Phytochemical Analysis*

Lycopene was extracted and quantified by following the methods given by Ranganna [26] and Rodriguez-Amaya and Kimura [27]. The phenolic compounds were extracted by refluxing 50 mL of 80% *v*/*v* aqueous ethanol for 3 h at 40 ◦C and then total phenolic content (TPC) was determined as per the procedure given by Tapia-Salazar et al. [28]. The total flavonoids content (TFC) was determined by the aluminum chloride colorimetric method given by Zhishen et al. [29] and the results were expressed as quercetin equivalent (QE) mg/100 g db.
