**4. Conclusions**

The following conclusions can be drawn on the basis of the obtained results:

– The blossoms of *Sambucus nigra* L from the Rhodope region have the highest antioxidant activity, total phenol content (49.2 ± 1 mgGAE/g) and total flavonoid content (18.6 ± 0.5 mgQE/g)]. The blossoms of *Sambucus nigra* L from the Dobrich region have the lowest antioxidant activity, total phenols and flavonoid content. Probably,

the higher altitude and lower temperature in mountains could influence the accumulation of secondary metabolites in blossoms of *Sambucus nigra* L., which improves the antioxidant potential of the samples.


The obtained results were valuable for the nutritional potential of elderberry blossoms as edible flowers with antioxidant potential.

**Author Contributions:** K.N. and I.M.-T. constructed and conceived the project, V.A. and G.G. designed the study. D.B., V.G., N.I. and I.Y. performed the study. V.A., I.M.-T. and N.P. analyzed the data and interpreted the results. K.N., G.G. and N.P. wrote the article. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research received no external funding.

**Institutional Review Board Statement:** Not applicable.

**Informed Consent Statement:** Not applicable.

**Data Availability Statement:** Not applicable.

**Acknowledgments:** Special thanks to the Medical University-Pleven for the financial support provided for the publication of the paper.

**Conflicts of Interest:** The authors declare no conflict of interest.
