*2.5. Measurement of Cytokine Levels*

We used enzyme-linked immunosorbent assay (ELISA) kits to analyze the following in PVAT: interleukin-6 (IL-6; BMS625; ThermoFisher Scientific), tumor necrosis factor α (TNFα; ab100785; abcam, Cambridge, UK), and adiponectin (RRP300; R&D Systems, Inc., Minneapolis, MN, USA). The optical density was read at 450 nm. The concentrations of IL-6, TNFα, and adiponectin were calculated in accordance with the standard curves.
