*2.7. Quantitative Real-Time PCR*

DC were cultured in the presence of HPP or IP (both 1000 μM) for 6 or 24 h. Detection of NAD(P)H dehydrogenase (quinone 1) (NQO1, accession number P15559) expression and glutathione reductase (GSR, accession number P00390) expression were measured using quantitative real-time PCR. RNA was first extracted using the High-Pure RNA Isolation Kit, followed by cDNA synthesis using the High-Capacity cDNA reverse transcription kit. iTaq Universal SYBR Green mastermix was used in the reaction along with relevant primers—the sequences are listed in Table 1. A Bio-Rad CFX96 Real-Time System was used to carry out the reaction. mRNA expression levels for genes of interest were quantified and normalized to the housekeeping (β-actin) mRNA levels.

**Table 1.** Primer sequences. Table containing the forward and reverse primer sequences for NQO1, GSR, and β-actin.


## *2.8. DC ELISA Experiments*

For detection of cytokines, DC were cultured in the presence of HPP or IP (500–1000 μM) for 6 h prior to stimulation with LPS (100 ng/mL) for 24 h. Concentrations of IL-12p70, IL-23, TNF, IL-6, and IL-10 were quantified from supernatants by ELISA as per the manufacturers' protocols.
