**2. Materials and Methods**

## *2.1. Mice*

Female BALB/c mice (six- to eight-week-old) were purchased from DILAVE Laboratories (Uruguay). Gp91phox- knockout (B6.129S-Cybbtm1Din/J) mice were purchased from Jackson Laboratory (USA) and maintained at UATE, Institut Pasteur Montevideo. Six to eight BALB/c, gp91phox-, and C57BL/6 littermates (controls for gp91phox- mice) were used per experiment. Animals were kept in the animal house (URBE, School of Medicine, UdelaR, Uruguay), with water and food supplied *ad libitum*. Mouse handling, care, and experiments were carried out in compliance with institutional guidelines and regulations from the National Committee on Animal Research (Comisión Nacional de Experimentación Animal, CNEA, https://www.cnea.gub.uy/, accessed on 12 November 2021, National Law 18.611, Uruguay). Procedures involving animals were approved by the Universidad de la República's Committee on Animal Research (Comisión Honoraria de Experimentación Animal, CHEA Protocol Number 07153-000817-18).
