*3.2. Effect of HO-1 Depletion on Crry Expression*

Reduced Crry staining was observed in kidney, liver and lung tissue sections from *Hmox1*−/<sup>−</sup> rats (Figure 2, Table 1). On the contrary, Crry could not be detected in spleen sections (Figure 2). In the kidney, reduced Crry expression was observed mainly in renal tubular cells and in glomerular endothelial and epithelial cells (Figure 2). A segmental staining along the capillary walls was also observed in glomeruli. Crry expression was substantially diminished in *Hmox1*−/<sup>−</sup> rats in both glomerular and tubular compartments. In the liver, Crry was located primarily in hepatocytes, with almost complete absence in liver tissue section obtained from HO-1 depleted rats. In WT liver sections, Crry immunostaining was also observed in sinusoid areas and was absent in *Hmox1*−/<sup>−</sup> rats. In the lung, Crry was localized primarily in the alveolar walls in lung tissue sections of WT rats, with no change of immunochemical staining in the lung tissue of *Hmox1*−/<sup>−</sup> compared to WT rats (Figure 2, Table 1).

**Figure 2.** Crry immunostaining of tissues obtained from WT and *Hmox1*−*/*<sup>−</sup> rats. Crry was detected in all tissues apart from spleen. Reduced Crry protein staining was demonstrated in kidney, liver and lung tissue section with no apparent changes in spleen tissue sections. Magnification ×200.

## *3.3. Effect of HO-1 Absence on CD59 Expression*

CD59 staining was not detected in any of the tissues examined (Figure 3, Table 1). Lung *Hmox1*−*/*<sup>−</sup> tissue exhibited some areas of staining, but this was nonspecific.

**Figure 3.** CD59 immunostaining of WT and *Hmox1*−*/*<sup>−</sup> rat tissue sections. CD59 was undetectable in all tissues examined. Magnification ×200.
