*2.2. JAR Spheroid Formation*

For the spheroid formation assay, we seeded 100 <sup>µ</sup>L of JAR cells at a density of 1×10<sup>5</sup> cells/mL in each well of an ultra-low attachment 96-well round bottom plate (Corning, NY, USA). Cells were incubated overnight at 37 ◦C in a 5% CO<sup>2</sup> incubator. During this incubation, JAR cells formed spheroids of 50–200 µm in diameter via natural aggregation. The aggregate diameter of the spheroids was measured by CellSens standard software (Olympus, Tokyo, Japan).
