*2.11. Toxicity Test*

C57BL6/N mice (6 weeks old) were intravenously injected with OVA mRNA-loaded A-11-LNPs at two doses of 0.03 mg mRNA/kg on days 0 and 3. Blood was obtained 24 h after the last dose and processed into plasma using heparin. Alanine transferase (ALT), aspartate transferase (AST), total bilirubin (T-BIL), lactate dehydrogenase (LDH), blood urea nitrogen (BUN), and creatinine (CRE) levels in plasma were measured at Oriental yeast Co., Ltd. (Shiga, Japan).
