*2.3. Particle-TLR Agonist Association Studies*

PLA iNPs (concentrations of iNPs as described in the results) were incubated with 1 µg/mL ODN 1668 FITC or 1 µg/mL FITC LPS in sterile DPBS containing 10% heat inactivated fetal bovine serum (FBS). These samples were incubated for 1 h at 37 ◦C at 5% CO<sup>2</sup> and vortexed every 10 min. Following incubation, the solutions were centrifuged for 5 min at 12,000× *g* to pellet iNPs then the supernatant was transferred to black 96-well plates to measure fluorescence at 525 nm with the Molecular Devices (San Jose, CA, USA) SpectraMax iD3 Microplate Reader.
