*2.13. Immune Cell Assay*

Spleens, lymph nodes and tumors were collected after sacrificing the animals and processed into single cell suspensions for analysis of CD4<sup>+</sup> cell, CD8<sup>+</sup> cell, Treg. The whole procedure of the mentioned immune cell processing was carried out according to the specifications of the test kit. Analysis of the stained immune cells was performed by flow cytometry.
