*2.4. Cell Culture*

Both the murine colorectal carcinoma (CT26) and the murine mammary adenocarcinoma cell line (4T1) were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). CT26 cells and 4T1 cells were cultured in RPMI supplemented with 10% (*v*/*v*) FBS, 100 units penicillin/mL, and 100 mg streptomycin/mL and maintained in an incubator under a humidified atmosphere with 5% CO<sup>2</sup> at 37 ◦C. The different in vitro tests in this study were performed using either 12- or 96-well microplates, with <sup>4</sup> <sup>×</sup> <sup>10</sup><sup>4</sup> CT26 or <sup>1</sup> <sup>×</sup> <sup>10</sup><sup>4</sup> 4T1 cells per well.
