*2.6. Characterization of RNA-Loaded LNPs*

The ζ-average size, polydispersity index (PdI), and ζ-potential of the LNPs were measured by means of a Zetasizer Nano ZS ZEN3600 instrument (Malvern Instruments, Worchestershire, UK). The encapsulation efficiency and total concentration of RNA were measured by a Ribogreen assay, as described previously [41].
