*2.1. Materials*

Roswell Park Memorial Institute medium (RPMI 1640), fetal bovine serum (FBS), penicillin, streptomycin, and trypsin were all purchased from Gibco, Carlsbad, CA, USA. Ethanol (99.3◦ GL) and glucose were all purchased from J.T. Trypan blue and dimethylsulfoxide (DMSO 99.5%); Trypan blue, 40 ,6-diamidino-e-fenilindol (DAPI), Annexin V (AnV), and propidium iodide (PI) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Aluminum-phthalocyanine (AlPc) was purchased from Aldrich Chemical Company (St. Louis, MO, USA). PBS was purchased from Pinhais, Paraná, Brazil. Enzyme-linked immunosorbent assay (ELISA) kit for high mobility group box-1 (HMGB1) was purchased in IBL International GmbH (Hamburg, Germany). ELISA kit for heat shock proteins (HSP)-70 and <sup>−</sup>90, primary anti-mouse/human antibody against CRT, and Alexa Fluor® 488 Goat Anti-Mouse (IgG) secondary antibodies (IgG488) were purchased from ABCAM (Cambridge, UK). ELISA kit for interleukin-1beta (IL-1β) was purchased from Life Technologies (Carlsbad, CA, USA).
