*2.6. Flow Cytometry*

Cell staining was conducted according to BioLegend protocols for flow cytometry. Flow cytometry data were collected using a Becton Dickinson LSR II or Becton Dickinson Canto II flow cytometer. Analysis was performed using FCS Express 7 (De Novo Software, Glendale, CA, USA). FcR blocking was performed with the anti-CD16/32 antibody prior to staining. Viability was assessed with 40 ,6-diamidino-2-phenylindole dilactate (DAPI) as an exclusion dye for iNP and TLR agonist studies.
