*2.2. Mice and Cell Cultures*

E.G7-OVA cells, generated by transducing the chicken OVA gene into the murine lymphoma cell line EL4, were obtained from the American Type Culture Collection (Manassas, VA, USA). E.G7-OVA cells were grown in Roswell Park Memorial Institute (RPMI) 1640 (Sigma Aldrich, St. Louis, MO, USA) supplemented with 50 µM of β-mercaptoethanol, 10% fetal calf serum, 10 mM 2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic acid (HEPES), 1 mM sodium pyruvate and 100 units/mL of penicillin/streptomycin. Cells were cultured at 37 ◦C in a 5% CO<sup>2</sup> incubator.

C57BL/6N mice (female, 6 weeks of age) were purchased from Japan SLC (Shizuoka, Japan).
