**5. Conclusions**

Our present work has characterized the detoxification gene families of *T. yunanensis* by transcriptome, together with bioinformatics-based analyses and molecular strategies of qPCR. Our study first lead to the identification of 113 genes associated with detoxification, with as many as 51 CYPs. Further, expression profile studies provided reference data for these genes to explore their potential roles in the detoxification of plant secondary metabolites. Together, this study has complemented the information of detoxification gene families in *T. yunanensis* and will allow for target experiments to screen potential attractants or repellents and to develop novel pest control strategies for controlling this beetle and protect the balance of the forest ecosystem.

**Supplementary Materials:** The following supporting information can be downloaded at: https: //www.mdpi.com/article/10.3390/10.3390/d14010023/s1, Table S1: The primers designed for qPCR analysis; Table S2: Protein sequence of Tomicus yunnanensis.

**Author Contributions:** Conceptualization, W.L. and N.Z.; methodology, B.Y. and N.L.; software, J.Z. and Z.L.; validation, W.L., S.Z. and N.Z.; formal analysis, N.L.; investigation, J.Z. and Z.L.; resources, W.L. and B.Y.; data curation, J.Y.; writing—original draft preparation, W.L.; writing—review and editing, W.L. and N.Z.; visualization, N.Z.; supervision, N.Z.; project administration, N.Z.; funding acquisition, N.Z. All authors have read and agreed to the published version of the manuscript.

**Funding:** The research was supported by the National Natural Science Foundation of China (31760210), the Science Research Foundation of Yunnan Provincial Department of Education Project (2021Y269), and Key Project of Yunnan Applied Basic Research Program (grand No.202101AS070009; 2018FG001-010).

**Institutional Review Board Statement:** Not applicable.

**Data Availability Statement:** All Data are provided within the paper and supplementary material.

**Conflicts of Interest:** All the authors declare that they have no conflict of interest.
