*2.2. In Vitro Experiments*

Phase II metabolites were generated by incubation of liver microsomes (rat, pig, human) with the respective phase I metabolites 3- OH and bAE. Subsequently characterization was carried out by a HPLC-qTOF-MS approach. Experimental conditions were set for glucuronic and sulfuric acid conjugation and experimental settings are described below. As sulfuric acid conjugation was not successful, further sample preparation focuses on glucuronidation. Details for sulfuric acid conjugation are described in Supporting Information Table S1.
