*4.1. Animals*

Male NZO mice (*n* = 22, NZO/BomHIDife genetic background) from Max-Rubner-Laboratory, German Institute of Human Nutrition Potsdam-Rehbrücke (Nuthetal, Germany) were used. These mice had increased weight (45.90 ± 4.11g b.w) and were previously characterized [7]. Mice were held in specific-pathogen-free (SPF) condition, in a 12:12 h

light–dark cycle, with free access to food and drinking water. All experimental procedures were approved by the Berlin Animal Review Board, Berlin, Germany and followed the restrictions in the Berlin State Office for Health and Social Affairs (LaGeSo) [37]. All experiments were performed in accordance with ARRIVE guidelines [38].
