*4.7. Statistics*

Statistical analyses were performed using GraphPad Prism version 9.4.1 (GraphPad Software, San Diego, CA, USA). All data are presented as mean ± S.E.M. For comparison of the means between two groups, data were analyzed by Student's 2-tailed *t*-test. Differences of the means for more than two groups containing two variables were analyzed using 2-way ANOVA. Posthoc analysis was performed with Tukey's test. A *p*-value of less than 0.05 was considered significant.

**Supplementary Materials:** The following supporting information can be downloaded at: https: //www.mdpi.com/article/10.3390/ijms232315310/s1.

**Author Contributions:** Conceptualization, R.H.M. and W.W.C.; methodology, R.H.M., S.G. and W.W.C.; software, W.W.C.; validation, R.H.M., S.G. and W.W.C.; formal analysis, R.H.M., S.G., E.A.O. and W.W.C.; investigation, S.G. and W.W.C.; resources, R.H.M. and W.W.C.; data curation, S.G. and W.W.C.; writing, R.H.M., S.G., E.A.O. and W.W.C.; project administration, W.W.C.; funding acquisition, R.H.M. and S.G. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research was funded by a Genentech Fellowship grant to S.G.

**Institutional Review Board Statement:** This study was conducted in compliance with established guidelines and prevailing protocol (S01754) as approved by the Institutional Animal Care and Use Committee (IACUC) at the University of California, San Diego, in accordance with the National Institutes of Health.

**Informed Consent Statement:** Not applicable.

**Data Availability Statement:** The authors confirm that the data supporting the findings of this study are available within the article and its Supplementary Materials. Additional raw data supporting the findings of this study are available from the corresponding author (R.H.M.) on request.

**Acknowledgments:** We thank Jianhua Shao and the UCSD Pediatric Diabetes Research Center for the use of EchoMRI-100™. We acknowledge contributions from Ping Zhou, Sheng Hao, and Ronghao Zheng in the generation of supplemental data.

**Conflicts of Interest:** The authors declare no conflict of interest.
