*2.4. GH Mitigates Lipolytic Enzymes in CKD Mice*

Elevated lipolysis is important for adipose tissue wasting in cachexia [21]. We investigated the molecular basis for the loss of adipose tissue in CKD mice. Inguinal WAT gene expression and protein content of adipose triglyceride lipase (ATGL) was significantly increased in vehicle-treated CKD mice (Figure 3A,B). Inguinal WAT gene expression and protein content of hormone-sensitive lipase (HSL) was not different among groups of mice (Figure 3C,D). However, phosphorylated HSL Ser552 protein content in inguinal WAT, a surrogate marker for protein kinase A-activated lipolysis, was five-fold higher in vehicletreated CKD mice compared to control mice (Figure 3E). Importantly, GH significantly decreased inguinal WAT gene expression and protein content of ATGL as well as protein content of phosphorylated HSL in CKD mice.
