*2.4. Gene Loss/Duplication Analysis of the NLR Genes*

In order to identify the gene duplication/loss events during the speciation of the five Arecaceae species, the NLR gene phylogenetic tree was reconciled with the species tree using Notung-2.9 software [29]. The types of NLR gene duplication within a genome were determined using the MCScanX package [30] based on a pair-wise all-against-all blast of protein sequences.
