**5. Conclusions**

In the present study, the disease symptoms and resistance for BW were evaluated by the inoculation with *R. solanacearum* using 94 F2-segregating populations. Using GBS, 628,437 SNPs were identified, and the filtered 1550 SNP were subsequently constructed into the genetic linkage map displaying 16 LG. QTL analysis revealed that *pBWR-1* QTL was located on chromosome 01, and the number of 31 candidate genes were identified in the significant QTL regions. Importantly, the identified four genes encode defense resistanceassociated proteins, such as CC-NB-LRR family protein, NB-ARC domain-containing protein, and Thaumatin-like proteins. Our finding will contribute to deep insights into the information for developing SNP markers associated with BW-resistant QTL as well as for developing BW-resistant cultivars in pepper breeding programs.

**Supplementary Materials:** The following are available online at https://www.mdpi.com/article/ 10.3390/horticulturae8020115/s1, Supplementary Figure S1: Distribution of the disease index after inoculation with *Ralstonia solanacearum*; Table S1: Genotyping-by-sequencing (GBS) statistics for 96 samples; Table S2: Single-nucleotide polymorphism (SNP) filtering criteria.

**Author Contributions:** Conceptualization, J.-B.Y.; methodology, S.-Y.C., J.-W.D., S.-C.H. and K.-H.L.; investigation, S.-Y.C., J.-W.D., S.-C.H. and K.-H.L.; writing—original draft preparation, S.-Y.C. and K.L.; writing—review and editing, K.L., M.-C.C. and E.-Y.Y.; visualization, K.L. and E.-Y.Y.; supervision, M.-C.C. and J.-B.Y. All authors have read and agreed to the published version of the manuscript.

**Funding:** This study was supported by a grant from Golden Seed Project (Project No.: 213006-05- 05-SB630 "Development of molecular markers for Bacterial wilt resistance and high-pungency in chili pepper").

**Institutional Review Board Statement:** Not applicable.

**Informed Consent Statement:** Not applicable.

**Data Availability Statement:** The datasets presented in this study are available upon request to the corresponding author.

**Acknowledgments:** The authors thank Sung-Hwan Jo at SEEDERS Inc., Republic of Korea, for assistance via intellectual discussions.

**Conflicts of Interest:** The authors declare no conflict of interest.
