*Article* **QTL Mapping of Resistance to Bacterial Wilt in Pepper Plants (***Capsicum annuum***) Using Genotyping-by-Sequencing (GBS)**

**Soo-Young Chae 1,†, Kwanuk Lee 2,†, Jae-Wahng Do 1, Sun-Cheul Hong 1, Kang-Hyun Lee 1, Myeong-Cheoul Cho 2, Eun-Young Yang 2,\* and Jae-Bok Yoon 1,\***


**Abstract:** Bacterial wilt (BW) disease, which is caused by *Ralstonia solanacearum*, is one globally prevalent plant disease leading to significant losses of crop production and yield with the involvement of a diverse variety of monocot and dicot host plants. In particular, the BW of the soil-borne disease seriously influences solanaceous crops, including peppers (sweet and chili peppers), paprika, tomatoes, potatoes, and eggplants. Recent studies have explored genetic regions that are associated with BW resistance for pepper crops. However, owing to the complexity of BW resistance, the identification of the genomic regions controlling BW resistance is poorly understood and still remains to be unraveled in the pepper cultivars. In this study, we performed the quantitative trait loci (QTL) analysis to identify genomic loci and alleles, which play a critical role in the resistance to BW in pepper plants. The disease symptoms and resistance levels for BW were assessed by inoculation with *R. solanacearum*. Genotyping-by-sequencing (GBS) was utilized in 94 F2 segregating populations originated from a cross between a resistant line, KC352, and a susceptible line, 14F6002-14. A total of 628,437 single-nucleotide polymorphism (SNP) was obtained, and a pepper genetic linkage map was constructed with putative 1550 SNP markers via the filtering criteria. The linkage map exhibited 16 linkage groups (LG) with a total linkage distance of 828.449 cM. Notably, QTL analysis with CIM (composite interval mapping) method uncovered *pBWR-1* QTL underlying on chromosome 01 and explained 20.13 to 25.16% by R<sup>2</sup> (proportion of explained phenotyphic variance by the QTL) values. These results will be valuable for developing SNP markers associated with BW-resistant QTLs as well as for developing elite BW-resistant cultivars in pepper breeding programs.

**Keywords:** pepper plant; *Ralstonia solanacearum*; single-nucleotide polymorphism (SNP); genotypingby-sequencing (GBS); quantitative trait loci (QTL); pepper breeding
