*Article* **Effects of Vertically Heterogeneous Soil Salinity on Genetic Polymorphism and Productivity of the Widespread Halophyte** *Bassia prostrata*

**Elena Shuyskaya 1,\*, Kristina Toderich 2, Alexander Kolesnikov 3, Maria Prokofieva <sup>1</sup> and Marina Lebedeva <sup>4</sup>**


**Abstract:** Salinity is one of the environmental factors that affects both productivity and genetic diversity in plant species. Within the soil profile, salinity is a dynamic indicator and significantly changes with depth. The present study examined the effects of the vertical heterogeneity of soil salinity chemistry on the plant height, fresh and dry biomass accumulation, water content, level of genetic polymorphism, and observed and expected heterozygosity in seven populations of halophyte *Bassia prostrata* in natural habitats. Soil salinity ranged from slight (Ssalts = 0.11–0.25%) to extreme (Ssalts = 1.35–2.57%). The main contributors to salinity were Na+, Ca2+, and Mg2+. Multivariate analysis revealed that biomass accumulation is positively affected by moderate/high salinity in 20–60 cm soil layers, which may be associated with the salt required for the optimal growth of the halophyte *B. prostrata.* The formation of seed genetic diversity is negatively affected by slight/moderate salinity in the 0–40 cm layers. An increase in divalent ion content can reduce genetic diversity and increase the local adaptation of *B. prostrata* to magnesium–calcium sulfate salinity. The effect of the in-depth distribution of soil salinity on productivity and genetic diversity may be related to seasonal variables during biomass accumulation (summer) and seed formation (autumn).

**Keywords:** plant performance; C4 species; heterozygosity; transient soil salinity; soil layers; desertification; arid regoins
