*2.7. Determination of Total Phenolic Content*

Total phenolic content was determined following Velioglu et al. [22]. Plant extracted solution (0.5 mL) was added to diluted Folin–Ciocalteu reagent (2 N, 5 mL), and then 4 mL of 1 M Na2CO3 and 1 mL water were added to the mixture. The leaf extracts were left to stand for 90 min at 37 ◦C, and then the phenolic content was determined by using colorimetry at 765 nm. The results were expressed as gallic acid equivalents per milligram (mg GAE g−<sup>1</sup> DW). The gallic acid solutions were prepared in methanol: water (50:50, *v/v*) as 0, 50, 100, 150, 200, and 250 mg mL−<sup>1</sup> for standard curve (R2 = 0.99).
