*2.6. Whole Plant Regeneration from Somatic Embryos*

To accelerate complete plant development from somatic embryos, the effects of Kn and IAA on further conversion of somatic embryos were studied. The somatic embryos were sub-cultured on the MS medium augmented with different concentrations of Kn (0.05–1.0 mg L−1) and IAA (0.1–1.0 mg L−1), either individually or in combination. After 8 weeks of culture, the average number of shoots and roots developed by somatic embryos were evaluated. All cultures were incubated at 10 ± 2 ◦C in a 16/8-h light/dark cycle with 60% RH. Each treatment consisted of 10 somatic embryos in 30 mL borosilicate culture vials and 150 mL conical flasks, and the experiment was repeated 3 times.
