2.2.5. ABTS Scavenging Activity

The ABTS (2,2 -azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)) scavenging activity was determined following Re et al. [37]. Before the experiment, 7 mM ABTS stock solution was mixed in a ratio of 1:1 with 2.45 mM potassium persulfate and incubated at room temperature for 16 h in the dark. The ABTS<sup>+</sup> solution was diluted with ethanol after incubation until the absorbance was detected at 0.700 ± 0.05 at 734 nm. The extract was then combined with 0.9 mL of diluted ABTS<sup>+</sup> solution. After 15 min, the absorbance of the reaction mixture was measured at 734 nm. As a control, Trolox was utilized. The ABTS scavenging activity was measured in milligrams of Trolox equivalent per gram of dry weight (mg TE/g DW).
