3.3.2. Experimental Design

The tested culture media were composed of two parts: the first part was a solidified MS62 culture medium supplemented with BAP (1 mg/L), which was covered with the second part, comprising 2 mL of liquid culture medium with the same composition and supplemented with 2,4-D (0.1 mg/L) and/or SA (0.1 mg/L). The entire experiment was set up in Petri dishes of 6 cm in diameter. The inoculum was fixed to the surface of the solidified culture medium, and a thin liquid culture medium covering the inoculum ensured rapid and uniform contact with the used signaling molecules.
