**5. Conclusions**

In summary, this study shows that brown seeds with a high germination percentage should be chosen as a source of sterile explants. The best sterilization method entailed 75% ethanol 60 s + 3.6% NaClO 8 min, placed in an MS medium with pH 8.0 for 7 days. At present, this method has been successfully applied to the seed sterilization of *S. aralocaspica* in vitro, and the pollution-free percentage can reach 100%, when ignoring the pollution caused by improper operation. The sterilization and cultivation method was successful for *S. aralocaspica* and may be also applied to other *Suaeda* species. In the future, other combinations of sterilization methods should be tested for *S. aralocaspica* seeds, and the exact mechanism of the sterilization effects needs to be fully understood.

**Supplementary Materials:** The following supporting information can be downloaded at: https: //www.mdpi.com/article/10.3390/horticulturae8040289/s1, Figure S1: The final germination percentage and contamination percentage of dimorphic seeds that washed with sterile water and incubated at different pH conditions, Table S1: 25 different treatments with NaClO as the secondary disinfector to sterilize brown seeds of *Suaeda aralocaspica,* Table S2: 25 different treatments with NaClO as the secondary disinfector to sterilize black seeds of *Suaeda aralocaspica*, Table S3: 25 different treatments with 0.1% HgCl2 as the secondary disinfector to sterilize brown seeds of *Suaeda aralocaspica*, Table S4: 25 different treatments with 0.1% HgCl2 as the secondary disinfector to sterilize black seeds of *Suaeda aralocaspica.*

**Author Contributions:** Conceptualization, L.W. and Y.S.; methodology, Y.S. and Y.H.; software, Y.S.; validation, L.W. and Y.S.; formal analysis, Y.S.; investigation, Y.S.; resources, L.W.; data curation, Y.S.; writing—original draft preparation, Y.S.; writing—review and editing, L.W., Y.S. and Y.H.; visualization, Y.S.; supervision, L.W.; project administration, L.W.; funding acquisition, L.W. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research was funded by the National Natural Science Foundation of China (grant number 32171514) and the Tianshan elite program of Xinjiang Uygur Autonomous Region (grant number Y970000333).

**Institutional Review Board Statement:** Not applicable.

**Informed Consent Statement:** Not applicable.

**Data Availability Statement:** The data presented in this study are available on request from the corresponding author.

**Conflicts of Interest:** The authors declare no conflict of interest.
