**5. Conclusions**

Our results revealed that shoot mass and length could be promoted by controlling light quality and intensity. However, the effect of light quality and intensity related to plant growth and development (stem diameter, shoot and root fresh weight, shoot and root dry weight, root length, root number, and leaf number) were not evident despite contrasting reports from other studies cited here. This leaves additional work to be performed to better address the effects of light sources, intensity, and quality on the in vitro growth and development of different banana varieties. Although not directly evaluated in this study, the number of in vitro shoots produced per explant were higher for some of the cultures grown under the LED lightings, with multiple shoots produced, while no shoots were formed in cultures grown under FL. Subsequent studies will address such parameters. Different responses as demonstrated between the two banana varieties (Musa 'Little Prince' and Musa 'Truly Tiny') indicated a genotype effect as affected by different light environments. LED lighting affected the relative chlorophyll content as well as stomata size in banana in vitro plantlets. Based on the responses for both banana varieties to the different light sources evaluated in this study, we suggest that LEDs at 90 μmol m−<sup>2</sup> s−<sup>1</sup> could be a suitable selection for the micropropagation of ornamental bananas.

**Author Contributions:** Conceptualization, W.A.V.; methodology, W.A.V., C.F., D.B. and P.M.C.B.; validation, W.A.V.; formal analysis, W.A.V.; investigation, C.F. and P.M.C.B.; resources, W.A.V. and D.B.; data curation, C.F.; writing—original draft preparation, C.F.; writing—review and editing, W.A.V., D.B. and P.M.C.B.; supervision, W.A.V. and D.B.; project administration, W.A.V. and D.B.; funding acquisition, W.A.V. All authors have read and agreed to the published version of the manuscript.

**Funding:** This research project was funded under the USAID-AREA project at the University of Florida, Project Number G000260- 60290000-201-3300-00118870.

**Institutional Review Board Statement:** Not applicable.

**Informed Consent Statement:** Not applicable.

**Data Availability Statement:** Not applicable.

**Acknowledgments:** This study was supported by the USAID-AREA project under the Department of Environmental Horticulture, Institute of Food and Agricultural Sciences, the University of Florida.

**Conflicts of Interest:** The authors declare no conflict of interest.
