2.1.5. Fresh and Dry Weight of Callus

Growth parameters were made in terms of an increase in the fresh and dry weights of cultured tissue. The cultured tissue was carefully taken from the culture vessel and cleaned of agar particles that had adhered at the point of contact. After that, the tissue was placed on pre-weighed aluminum foil and the weight was calculated using a single pan digital balance of fresh weight of callus which presented as gram (g). The tissues were oven-dried at 55 ◦C to a consistent weight on the same foils for estimation of their dry weight as milligrams (mg) after recording their fresh weight.
