*3.5. Flow Cytometric Profile of Plants*

Flow cytometric examination of in vitro grown plants is a considerably faster and more reliable technique of ensuring ploidy genetic uniformity. Thus, comparative ploidy levels were determined in this study using a Muse Cell Analyzer in isolated nuclei from *P. amboinicus* micropropagated plants and a parent plant. The histograms produced from the nuclei of all plant sources, as previously stated, reveal a unimodal fluorescence peak of nuclear DNA. The cytometric profile indicated that all plant sources had nearly identical G0/G1 positions (Figure 4). As a result of the experimental findings, it was discovered that there were no differences between their usual fluorescence peak in the histogram of all plant sources, indicating that in vitro propagated *P. amboinicus* plants-maintained homogeneity of ploidy level, which ensures the successful clonal multiplication of this plant without compromising the genetic integrity.

**Figure 4.** Flow cytometric profiles of tissue culture raised plants (**A**) and donor plants (**B**) of *Plectranthus amboinicus*.
